[壳聚糖膜上固定化脲酶活性的X射线微分析]

[X-ray microanalysis of the activity of immobilized urease on chitosan membrane].

作者信息

Ma Xiao-li, Yao Zi-hua

机构信息

College of Chemistry and Environmental Science, Hebei University, Hebei Analytical Science and Technology Key Lab, Baoding 071002, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Mar;25(3):456-9.

PMID:16013332

Abstract

The localization of the activity of immobilized urease on chitosan membrane was studied by X-ray microanalysis. BaCl2 and urea were selected as the capture and substrate respectively. The substrate was hydrolyzed by immobilized urease to produce NH3 and CO2 in Tris-HCl buffer (pH 7.0), and the latter was captured by BaCl2 to form precipitate. The precipite was deposited on the active site of immobilized urease. It is shown that the method is practicable and reliable. The optimum condition for the localization of activity of immobilized urease was studied.

摘要

通过X射线微分析研究了固定在壳聚糖膜上的脲酶活性的定位。分别选用BaCl₂和尿素作为捕获剂和底物。在Tris-HCl缓冲液（pH 7.0）中，底物被固定化脲酶水解产生NH₃和CO₂，后者被BaCl₂捕获形成沉淀。沉淀沉积在固定化脲酶的活性部位上。结果表明该方法可行且可靠。研究了固定化脲酶活性定位的最佳条件。