Mohanna Pari-Naz, Terenghi Giorgio, Wiberg Mikael
Blond McIndoe Laboratories, The University of Manchester, Manchester, UK.
Scand J Plast Reconstr Surg Hand Surg. 2005;39(3):129-37. doi: 10.1080/02844310510006295.
Two to four cm nerve gaps in the rabbit common peroneal nerve were bridged with poly-3-hydroxybutyrate (PHB) conduits containing either glial growth factor (GGF) (PHB-GGF) or alginate matrix (PHB-ALG), and with empty PHB conduit (E-PHB). PHB-GGF significantly increased nerve regeneration up to 63 days following repair of long nerve gaps and the regeneration was sustained long term leading to motor organ reinnervation. At 120 days postoperatively, GGF addition significantly increased the quantity of Schwann cell and axonal regeneration compared to those in control conduits. In PHB-GGF conduits there were more minifascicles of myelinated fibres compared to the controls. The distal nerve of PHB-GGF and E-PHB conduits showed greater regeneration than that of PHB-ALG grafts, although all distal nerves contained fewer myelinated fibres than grafted conduits. Consistently, PHB-GGF conduits significantly reduced the muscle mass percentage loss compared to controls. In conclusion, GGF-containing conduits promoted sustained axonal regeneration and improved target muscle reinnervation.
用含有神经胶质生长因子（GGF）的聚3-羟基丁酸酯（PHB）导管（PHB-GGF）、藻酸盐基质（PHB-ALG）的PHB导管以及空的PHB导管（E-PHB）桥接兔腓总神经中2至4厘米的神经间隙。在修复长神经间隙后的63天内，PHB-GGF显著促进了神经再生，且再生长期持续，导致运动器官重新获得神经支配。术后120天时，与对照导管相比，添加GGF显著增加了雪旺细胞数量和轴突再生。与对照组相比，PHB-GGF导管中有更多有髓纤维的小束。尽管所有远端神经中的有髓纤维都比移植导管中的少，但PHB-GGF和E-PHB导管的远端神经显示出比PHB-ALG移植物更大的再生。同样，与对照组相比，PHB-GGF导管显著降低了肌肉质量百分比损失。总之，含GGF的导管促进了轴突的持续再生并改善了靶肌肉的重新神经支配。
