采用高效液相色谱-二极管阵列检测法对葡萄和葡萄酒中的嘧菌酯、醚菌酯和肟菌酯杀菌剂进行筛查。

Screening of grapes and wine for azoxystrobin, kresoxim-methyl and trifloxystrobin fungicides by HPLC with diode array detection.

作者信息

De Melo Abreu Susana, Correia Manuela, Herbert Paulo, Santos Lúcia, Alves Arminda

机构信息

LEPAE-DEQ, Faculdade de Engenharia da Universidade do Porto, Portugal.

出版信息

Food Addit Contam. 2005 Jun;22(6):549-56. doi: 10.1080/02652030500137918.

DOI:10.1080/02652030500137918

PMID:16019828

Abstract

The Quinone outside Inhibitors (QoI) are one of the most important and recent fungicide groups used in viticulture and also allowed by Integrated Pest Management. Azoxystrobin, kresoxim-methyl and trifloxystrobin are the main active ingredients for treating downy and powdery mildews that can be present in grapes and wines. In this paper, a method is reported for the analysis of these three QoI-fungicides in grapes and wine. After liquid-liquid extraction and a clean-up on commercial silica cartridges, analysis was by isocratic HPLC with diode array detection (DAD) with a run time of 13 min. Confirmation was by solid-phase micro-extraction (SPME), followed by GC/MS determination. The main validation parameters for the three compounds in grapes and wine were a limit of detection up to 0.073 mg kg(-1), a precision not exceeding 10.0% and an average recovery of 93% +/- 38.

摘要

醌外抑制剂（QoI）是葡萄栽培中使用的最重要且最新的杀菌剂类别之一，也是综合虫害管理所允许使用的。嘧菌酯、醚菌酯和肟菌酯是用于防治葡萄和葡萄酒中可能出现的霜霉病和白粉病的主要活性成分。本文报道了一种分析葡萄和葡萄酒中这三种QoI杀菌剂的方法。经液液萃取并在商用硅胶柱上净化后，采用等度高效液相色谱法（HPLC）和二极管阵列检测（DAD）进行分析，运行时间为13分钟。通过固相微萃取（SPME），随后进行气相色谱/质谱（GC/MS）测定来进行确证。葡萄和葡萄酒中这三种化合物的主要验证参数为：检测限高达0.073 mg kg⁻¹，精密度不超过10.0%，平均回收率为93%±38。