在小鼠模型中表达恶性肿瘤特异性抗原MAGE-A1表位的噬菌体展示病毒粒子的潜力。

The potential of phage display virions expressing malignant tumor specific antigen MAGE-A1 epitope in murine model.

作者信息

Fang Jinbo, Wang Guiyun, Yang Qiong, Song Jinna, Wang Ye, Wang Li

机构信息

School of Life Sciences, Northeast Normal University, 5268 Renmin Street, Nanguan District, Changchun 130024, PR China.

出版信息

Vaccine. 2005 Sep 23;23(40):4860-6. doi: 10.1016/j.vaccine.2005.05.024.

DOI:10.1016/j.vaccine.2005.05.024

PMID:16029917

Abstract

The tumor specific antigen epitope melanoma antigen A1(161--169) (MAGE-A1(161--169)) was displayed on the major protein (p VIII) of the filamentous bacteriophage (fd). The immune responses of the phage display particles expressing MAGE-A1(161--169) in mice were studied. Using phage display particles as vaccine was effective in affording protection from tumor growth, inducing growth control of established tumors and prolonging survival of tumor-bearing mice. The hybrid phage particles elicited MAGE-A1(161--169) specific CTL responses, NK activity and DTH. Our results showed that the phage display particles might be a new vaccine candidate for tumor-associated antigen epitope in cancer immunotherapy.

摘要

肿瘤特异性抗原表位黑素瘤抗原A1（161 - 169）（MAGE - A1（161 - 169））展示于丝状噬菌体（fd）的主要蛋白（p VIII）上。研究了表达MAGE - A1（161 - 169）的噬菌体展示颗粒在小鼠体内的免疫反应。使用噬菌体展示颗粒作为疫苗可有效保护小鼠免受肿瘤生长，诱导已形成肿瘤的生长控制并延长荷瘤小鼠的生存期。杂交噬菌体颗粒引发了MAGE - A1（161 - 169）特异性CTL反应、NK活性和迟发型超敏反应。我们的结果表明，噬菌体展示颗粒可能是癌症免疫治疗中肿瘤相关抗原表位的一种新型疫苗候选物。