Chen Qing, Rehman S, Smant G, Jones John T
Plant-Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK.
Mol Plant Microbe Interact. 2005 Jul;18(7):621-5. doi: 10.1094/MPMI-18-0621.
RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted beta-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the beta-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.
RNA干扰（RNAi）已被广泛用作研究基因功能的工具，最近还描述了一种可用于植物寄生线虫的方法。在这里，我们使用一种改良方法来分析马铃薯胞囊线虫罗氏 Globodera rostochiensis 分泌的β-1,4-内切葡聚糖酶的功能，这是对植物寄生线虫致病性蛋白的首次体内功能分析。β-1,4-内切葡聚糖酶的敲除降低了线虫侵入根的能力。我们还使用RNAi表明，gr-ams-1，一种主要感觉器官（化感器）分泌的蛋白质，对宿主定位至关重要。
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