[构建含编码p30基因的抗弓形虫单价及复合核酸疫苗]
[Construction of monovalent and compound nucleic acid vaccines against Toxoplasma gondii with gene encoding p30].
作者信息
Yang Ting-ting, He Shen-yi, Jiang Hua, Gu Qin-min, Cong Hua, Zhou Huai-yu, Zhang Jia-qin, Li Ying, Zhao Qun-li
机构信息
Department of Parasitology, Medical College of Shandong University, Jinan 250012, China.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Feb 28;23(1):14-7.
OBJECTIVE
To construct a monovalent gene vaccine pcDNA3.1-p30 and a compound gene vaccine pcDNA3.1-p30-ROP2 and assess the protective effect of the two vaccines against Toxoplasma gondii.
METHODS
The sequences encoding p30 and ROP2 were amplified from the genomic DNA of T. gondii RH strain by polymerase chain reaction (PCR) and inserted into eukaryotic vector pcDNA3.1 to construct pcDNA3.1-p30 and pcDNA3.1-p30-ROP2. Mice were injected with the recombinant plasmid to observe the immunoprotectivity of the nucleic acid vaccine by using ELISA for detection of total IgG and observing the survival time after tachyzoites challenge.
RESULTS
The recombinant plasmids pcDNA3.1-p30 and pcDNA3.1-p30-ROP2 were constructed. Mice in pcDNA3.1-p30-ROP2 group showed higher IgG (P < 0.05) and survived longer than those in pcDNA3.1-p30 group (P < 0.01) after challenged with T. gondii.
CONCLUSION
Compound vaccine of genes from different stages of T. gondii elicits stronger immunoprotectivity in mice than a single gene vaccine.
目的
构建单价基因疫苗pcDNA3.1-p30和复合基因疫苗pcDNA3.1-p30-ROP2，并评估这两种疫苗对弓形虫的保护作用。
方法
通过聚合酶链反应（PCR）从弓形虫RH株基因组DNA中扩增编码p30和ROP2的序列，并将其插入真核载体pcDNA3.1中构建pcDNA3.1-p30和pcDNA3.1-p30-ROP2。给小鼠注射重组质粒，通过ELISA检测总IgG并观察速殖子攻击后的存活时间，以观察核酸疫苗的免疫保护作用。
结果
构建了重组质粒pcDNA3.1-p30和pcDNA3.1-p30-ROP2。用弓形虫攻击后，pcDNA3.1-p30-ROP2组小鼠的IgG水平更高（P<0.05），存活时间比pcDNA3.1-p30组更长（P<0.01）。
结论
来自弓形虫不同阶段的基因复合疫苗在小鼠中引发的免疫保护作用比单一基因疫苗更强。
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