大鼠巨细胞病毒主要立即早期区域两个反义转录本的鉴定与特征分析

Identification and characterization of two antisense transcripts from the major immediate early region of rat cytomegalovirus.

作者信息

van Cleef K W R, Blok M J, Savelkouls K G M, Grauls G E L M, Bruggeman C A, Vink C

机构信息

Department of Medical Microbiology, Cardiovascular Research Institute Maastricht (CARIM), University of Maastricht, Maastricht, The Netherlands.

出版信息

Arch Virol. 2005 Dec;150(12):2593-9. doi: 10.1007/s00705-005-0566-1. Epub 2005 Aug 1.

DOI:10.1007/s00705-005-0566-1

PMID:16052287

Abstract

We have identified and characterized two antisense transcripts from the rat cytomegalovirus (RCMV) major immediate early (MIE) region. These transcripts, designated IE-AS1 and IE-AS2, are complementary to part of the sense IE1 transcript. The IE-AS transcripts were first detected in peripheral blood leukocytes (PBL) of RCMV-infected rats at 7 days post-infection (pi) in the absence of IE1 transcription. Nevertheless, both the IE1 and IE-AS transcripts were found at the same time in the salivary glands of RCMV-infected rats at 7 and 120 days pi as well as in RCMV-infected rat embryo fibroblasts (REFs) at 48 h pi.

摘要

我们已经鉴定并表征了来自大鼠巨细胞病毒（RCMV）主要立即早期（MIE）区域的两种反义转录本。这些转录本被命名为IE-AS1和IE-AS2，与有义IE1转录本的一部分互补。IE-AS转录本首次在感染RCMV的大鼠外周血白细胞（PBL）中于感染后7天（pi）被检测到，此时不存在IE1转录。然而，在感染RCMV的大鼠唾液腺中，在感染后7天和120天pi以及在感染RCMV的大鼠胚胎成纤维细胞（REFs）中于感染后48小时pi时，同时发现了IE1和IE-AS转录本。