前列环素（PGI2）对抗凝血酶III和肝素使凝血酶、因子Xa及纤溶酶失活的影响。

Effects of PGI2 on the inactivation of thrombin, factor Xa, and plasmin by antithrombin-III and heparin.

作者信息

Blaskó G

出版信息

Prostaglandins. 1979 Jul;18(1):3-9. doi: 10.1016/s0090-6980(79)80017-9.

Abstract

The influence of PGI2 on the activity and on the inactivation of enzymes participating in blood coagulation (thrombin and Factor Xa) and fibrinolysis (plasmin) were investigated. According to the results PGI2 has no effect on the activity of Factor Xa and plasmin nor on the inactivation of these enzymes by antithrombin-III in the absence and presence of heparin at a concentration of PGI2 up to 400 micrograms/ml. An acceleration of the inactivation of thrombin by antithormbin-III was found in the presence of PGI2 within a concentration of 100-400 micrograms/ml without any effect on the heparin-accelerated inactivation of thrombin by antithrombin. We got similar results using clotting tests for the assay and the application of synthetic substrate for thrombin. This inactivation-accelerating effect of PGI2 on thrombin was only demonstratable at a concentration five magnitudes higher than that of the anti-aggregation effect on platelets.

摘要

研究了前列环素（PGI2）对参与血液凝固（凝血酶和因子Xa）和纤维蛋白溶解（纤溶酶）的酶活性及失活的影响。结果表明，在PGI2浓度高达400微克/毫升时，无论有无肝素存在，PGI2对因子Xa和纤溶酶的活性以及抗凝血酶III对这些酶的失活均无影响。在100 - 400微克/毫升浓度范围内，发现PGI2存在时抗凝血酶III对凝血酶的失活有加速作用，而对肝素加速抗凝血酶对凝血酶的失活无影响。使用凝血试验进行测定以及应用凝血酶的合成底物时，我们得到了相似的结果。PGI2对凝血酶的这种失活加速作用仅在比其对血小板抗聚集作用高五个数量级的浓度下才表现出来。