Pleyer U, Mondino B J, Sumner H L
Jules Stein Eye Institute, Los Angeles, CA.
Invest Ophthalmol Vis Sci. 1992 Jun;33(7):2212-5.
The authors examined the effect of systemic administration of cobra venom factor (CVF) on hemolytic complement levels in guinea pig sera and corneas. Guinea pigs received repeated intraperitoneal injections of CVF. Sera and corneas were obtained before and 1, 2, 5, 8, 10, 12, 16, and 20 d after the initial injection of CVF. Total hemolytic complement activity was measured by lysis of sheep erythrocytes sensitized with rabbit antibodies. There was a significant decrease in serum complement levels at days 1, 2, 5, 8, and 10 after the initial injection of CVF. After this, complement levels rose to levels that exceeded preinjection values. Complement levels in corneas also declined after CVF injections with a significant decrease at days 2, 5, 8, and 10 and a return to normal thereafter. Corneal complement depletion and restoration lagged behind serum. Moreover, the rate of complement depletion and restoration was greater for serum than cornea. Light microscopic examination of representative corneas at each time did not show local tissue changes during decomplementation. This is first study (to the authors' knowledge) to document corneal complement depletion by systemic administration of CVF. Low-to-absent corneal complement levels were present 2 d after the initial injection of CVF and persisted for 6 d. This model could be used to study the influence of complement on traumatic and immunologic injuries to the cornea.
作者研究了全身注射眼镜蛇毒因子（CVF）对豚鼠血清和角膜中溶血补体水平的影响。豚鼠反复腹腔注射CVF。在首次注射CVF之前以及之后的第1、2、5、8、10、12、16和20天采集血清和角膜。通过兔抗体致敏的绵羊红细胞溶解来测量总溶血补体活性。首次注射CVF后的第1、2、5、8和10天，血清补体水平显著下降。在此之后，补体水平上升至超过注射前的值。CVF注射后角膜中的补体水平也下降，在第2、5、8和10天显著下降，此后恢复正常。角膜补体的消耗和恢复滞后于血清。此外，血清中补体的消耗和恢复速率比角膜更快。在补体缺失期间，对每个时间点的代表性角膜进行光学显微镜检查未发现局部组织变化。据作者所知，这是第一项记录通过全身注射CVF导致角膜补体缺失的研究。首次注射CVF后2天角膜补体水平低至缺乏，并持续6天。该模型可用于研究补体对角膜创伤和免疫损伤的影响。
