Fabjani Gerhild, Tong Dan, Wolf Andrea, Roka Sebastian, Leodolter Sepp, Hoecker Paul, Fischer Michael Bernhard, Jakesz Raimund, Zeillinger Robert
Department of Obstetrics and Gynecology, General Hospital of Vienna, Molecular Oncology Group, Waehringer Guertel 18-20, 5Q, A-1090 Vienna, Austria.
Oncol Rep. 2005 Sep;14(3):737-41.
Previously, the human high mobility group protein member HMGA2 mRNA was reported to be expressed in peripheral blood of patients with breast cancer, but not in healthy individuals. Expression of HMGA2 in blood was suggested to be an independent indicator of poor prognosis in metastatic breast cancer. These very promising findings propose HMGA2 as a potential marker for the detection of circulating tumor cells in peripheral blood. Therefore, we analyzed peripheral blood specimens from healthy controls and patients with breast tumors for HMGA2 expression using TaqMan real-time RT-PCR to test if HMGA2 is a suitable marker for the early detection of breast cancer and monitoring therapy response in peripheral blood. Furthermore, we examined the possible involvement of HMGA2 expression in invasion investigated by an in vitro invasion assay using established breast cell lines. HMGA2 expression was detected in peripheral blood of breast cancer patients as well as of healthy individuals. No significant association of HMGA2 expression with any clinical or histopathological data was apparent. However, there was a significant correlation of HMGA2 expression in invasive and non invasive breast cell lines (p=0.0056). Although, HMGA2 obviously contributes to invasion it is not a specific marker for the detection of circulating tumor cells in peripheral blood.
此前，据报道人类高迁移率族蛋白成员HMGA2 mRNA在乳腺癌患者的外周血中表达，但在健康个体中不表达。血液中HMGA2的表达被认为是转移性乳腺癌预后不良的独立指标。这些非常有前景的发现表明HMGA2是检测外周血中循环肿瘤细胞的潜在标志物。因此，我们使用TaqMan实时逆转录聚合酶链反应分析了健康对照者和乳腺肿瘤患者的外周血标本中HMGA2的表达情况，以检测HMGA2是否是早期检测乳腺癌和监测外周血治疗反应的合适标志物。此外，我们通过使用已建立的乳腺细胞系进行体外侵袭试验，研究了HMGA2表达在侵袭过程中的可能作用。在乳腺癌患者以及健康个体的外周血中均检测到了HMGA2的表达。HMGA2表达与任何临床或组织病理学数据均无明显关联。然而，HMGA2在侵袭性和非侵袭性乳腺细胞系中的表达存在显著相关性（p = 0.0056）。尽管HMGA2明显促进侵袭，但它不是检测外周血中循环肿瘤细胞的特异性标志物。
