Cooper Gail, Wilson Lisa, Reid Claire, Baldwin Dene, Hand Chris, Spiehler Vina
Cozart Bioscience Ltd, Abingdon, Oxfordshire, UK.
J Forensic Sci. 2005 Jul;50(4):928-32.
The purpose of these studies was to evaluate the performance characteristics of the Cozart Microplate Enzyme Immunoassay (EIA) for the determination of methadone in oral fluid from patients in a drug misuse treatment program. Oral fluid specimens were collected using the Cozart RapiScan Collection system from 198 donors who were receiving treatment for their addiction and were monitored for drug misuse. Oral fluid specimens were also collected from forty volunteer donors who were not drug users. The specimens were analyzed in the laboratory by EIA and then analysed for methadone and its main metabolite EDDP by gas chromatography-mass spectrometry (GC-MS). A total of 103 samples were confirmed positive for methadone. The Cozart Microplate EIA for d-Methadone in oral fluid using a cutoff of 30 ng/mL in diluted oral fluid had a sensitivity of 91.3% +/- 2.8% and a specificity of 100% +/- 1.0% vs. GC-MS.
这些研究的目的是评估Cozart酶标板酶免疫分析法（EIA）在测定药物滥用治疗项目中患者口腔液中美沙酮含量时的性能特征。使用Cozart RapiScan采集系统从198名接受成瘾治疗并接受药物滥用监测的捐赠者中采集口腔液样本。还从40名非吸毒志愿者捐赠者中采集口腔液样本。样本在实验室中通过酶免疫分析法进行分析，然后通过气相色谱 - 质谱联用仪（GC - MS）分析美沙酮及其主要代谢物EDDP。共有103个样本被确认为美沙酮阳性。在稀释口腔液中使用30 ng/mL的临界值时，用于口腔液中d - 美沙酮检测的Cozart酶标板酶免疫分析法相对于GC - MS的灵敏度为91.3%±2.8%，特异性为100%±1.0%。
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