Jin Gu-Lei, Wang Xu-Sheng, Zhu Jun
Institution of bioinformatics, Zhejiang University, Hangzhou 310029, China.
Yi Chuan Xue Bao. 2005 Jul;32(7):726-32.
Using two-step HMM (hidden markov model) scan strategy,eight 14-3-3-like proteins were identified by searching the Oryza sativa L. ssp. japonica protein database. From them four genes were newly detected in this study. We confined the genes expressing in Nipponbare by EST search. Expression analysis also showed each gene expressed diversely within any individual,this tends suggested specific function of particular gene. Alignment of amino acid sequences suggested that there could be isoform function of the specific residues. The analyses of gene structure and chromosome location indicated that rice genome contains both epsilon and no-epsilon forms of 14-3-3 proteins. In addition,we analyzed the evolution of the rice 14-3-3 protein family.
采用两步隐马尔可夫模型（HMM）扫描策略，通过搜索日本晴水稻蛋白质数据库，鉴定出8个类14-3-3蛋白。本研究中新检测到其中4个基因。我们通过EST搜索确定了在日本晴中表达的基因。表达分析还表明，每个基因在任何个体中表达都存在差异，这倾向于暗示特定基因具有特定功能。氨基酸序列比对表明特定残基可能存在同工型功能。基因结构和染色体定位分析表明，水稻基因组中同时存在ε型和非ε型14-3-3蛋白。此外，我们还分析了水稻14-3-3蛋白家族的进化。
