Carulli Giovanni, Canigiani Silvia, Volpini Maila, Ciancia Eugenio M, Galimberti Sara, Zucca Alessandra, Marini Alessandra, Buda Gabriele, Petrini Mario
Divisione di Ematologia, Dipartimento di Oncologia, dei Trapianti e delle Nuove Tecnologie in Medicina, Università, Ospedale S. Chiara, Pisa.
Recenti Prog Med. 2005 Jun;96(6):284-90.
A comparison of flow cytometry (FC) and bone marrow biopsy (BMB) to evaluate bone marrow infiltration was made in 114 patients suffering from B-cell non-Hodgkin's lymphomas (NHLs; 51 at diagnosis, 63 during post-therapy follow-up). The following parameters were indicative of bone marrow infiltration: altered surface k/l ratio; specific immunophenotypic pattern in particular NHLs (CLL, mantle cell lymphoma, hairy cell leukemia). FC and BMB agreed in 89.5% of cases (i.e. both showed 48 positive and 54 negative cases). In discordant cases (7.9%) and in cases not evaluable by FC (2.6%) IgH rearrangement and bcl-1 gene expression, both evaluated by PCR methods, were used to detect bone marrow infiltration with higher precision. These results show that a more complex analysis of bone marrow is needed to diagnose bone marrow infiltration, particularly in samples with minimal residual disease.
对114例B细胞非霍奇金淋巴瘤（NHL；51例诊断时，63例治疗后随访期间）患者进行了流式细胞术（FC）和骨髓活检（BMB）以评估骨髓浸润的比较。以下参数提示骨髓浸润：表面k/l比值改变；特定NHL（慢性淋巴细胞白血病、套细胞淋巴瘤、毛细胞白血病）中的特定免疫表型模式。FC和BMB在89.5%的病例中结果一致（即两者均显示48例阳性和54例阴性病例）。在不一致的病例（7.9%）和FC无法评估的病例（2.6%）中，通过PCR方法评估的IgH重排和bcl-1基因表达用于更精确地检测骨髓浸润。这些结果表明，需要对骨髓进行更复杂的分析以诊断骨髓浸润，尤其是在微小残留病样本中。
Zotero 插件