Niebrój-Dobosz I M
Department of Neurology, Polish Academy of Science, Warsaw.
J Neurol. 1992 May;239(5):267-9. doi: 10.1007/BF00810350.
The relationship between fluorescence intensity and binding of 1-anilino-naphthalene-8-sulphonate (ANS) to muscle plasmalemma in patients with Duchenne's muscular dystrophy (DD) and controls was studied. The fluorescence of ANS was markedly enhanced in DD as compared with controls. The magnitude of this enhancement was increased by monovalent and divalent cations; treatment of DD plasmalemma with trypsin caused an opposite effect. Treatment with phospholipase A and C altered the ANS fluorescence in DD and controls equally. These findings may indicate an increase of the hydrophobic character in the apolar-polar interface of DD plasmalemma. The relationship of these changes to a lack of dystrophin in DD remains to be established.
研究了杜兴氏肌营养不良症（DD）患者和对照组中1-苯胺基萘-8-磺酸盐（ANS）与肌肉质膜结合之间的荧光强度关系。与对照组相比，DD患者中ANS的荧光明显增强。一价和二价阳离子可增加这种增强的程度；用胰蛋白酶处理DD质膜会产生相反的效果。用磷脂酶A和C处理对DD患者和对照组的ANS荧光改变程度相同。这些发现可能表明DD质膜的非极性-极性界面处疏水性增加。这些变化与DD中肌营养不良蛋白缺乏的关系仍有待确定。
