从大肠杆菌细胞包涵体中分离、溶解、复性及色谱纯化人生长激素：一个案例研究 - Suppr

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Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.

作者信息

Singh Surinder M, Eshwari A N S, Garg Lalit C, Panda Amulya K

机构信息

Product Development Cell, National Institute of Immunology, New Delhi, India.

出版信息

Methods Mol Biol. 2005;308:163-76. doi: 10.1385/1-59259-922-2:163.

DOI:10.1385/1-59259-922-2:163

PMID:16082034

Abstract

摘要

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相似文献

Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.

Methods Mol Biol. 2005;308:163-76. doi: 10.1385/1-59259-922-2:163.

Solubilization and refolding of bacterial inclusion body proteins.

J Biosci Bioeng. 2005 Apr;99(4):303-10. doi: 10.1263/jbb.99.303.

Recombinant murine growth hormone from E. coli inclusion bodies: expression, high-pressure solubilization and refolding, and characterization of activity and structure.

Biotechnol Prog. 2010 May-Jun;26(3):743-9. doi: 10.1002/btpr.393.

Optimization of inclusion body solubilization and renaturation of recombinant human growth hormone from Escherichia coli.

Protein Expr Purif. 2000 Mar;18(2):182-92. doi: 10.1006/prep.1999.1179.

High throughput purification of recombinant human growth hormone using radial flow chromatography.

Protein Expr Purif. 2009 Nov;68(1):54-9. doi: 10.1016/j.pep.2009.05.014. Epub 2009 Jun 16.

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Nan Fang Yi Ke Da Xue Xue Bao. 2006 Aug;26(8):1083-6.

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J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Apr 15;818(1):23-7. doi: 10.1016/j.jchromb.2004.10.023.

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Methods Mol Biol. 2011;681:259-80. doi: 10.1007/978-1-60761-913-0_14.

Isolation, solubilization, refolding, and chromatographic purification of human growth hormone from inclusion bodies of Escherichia coli cells: a case study.

作者信息

Singh Surinder M, Eshwari A N S, Garg Lalit C, Panda Amulya K

机构信息

Product Development Cell, National Institute of Immunology, New Delhi, India.

出版信息

Methods Mol Biol. 2005;308:163-76. doi: 10.1385/1-59259-922-2:163.

DOI:10.1385/1-59259-922-2:163

PMID:16082034

Abstract

摘要