Munné Santiago, Chen Serena, Fischer Jill, Colls Pere, Zheng Xuezong, Stevens John, Escudero Tomas, Oter Maria, Schoolcraft Bill, Simpson Joe Leigh, Cohen Jacques
Reprogenetics, LLC, Hoboken, New Jersey, USA.
Fertil Steril. 2005 Aug;84(2):331-5. doi: 10.1016/j.fertnstert.2005.02.027.
To determine whether preimplantation genetic diagnosis (PGD) and transfer of euploid embryos would decrease spontaneous abortion rates in recurrent miscarriage (RM) patients.
Controlled clinical study.
In vitro fertilization centers and PGD reference laboratory.
PATIENT(S): Recurrent-miscarriage patients with three or more prior lost pregnancies with no known etiology.
INTERVENTION(S): Biopsy of a single blastomere from each day 3 embryo, followed by fluorescence in situ hybridization analysis.
MAIN OUTCOME MEASURE(S): The rate of spontaneous abortions in RM subjects undergoing PGD were compared with [1] their own a priori expectations and [2] a comparison group of women undergoing PGD for advanced maternal age (> or =35 years).
RESULT(S): Before PGD, RM patients had lost 87% (262/301) of their pregnancies, with an expected loss rate of 36.5%. After, they only lost 16.7% pregnancies. This difference was mostly due to reduction in pregnancy loss in the > or =35-years age subgroup, to 12% from an expected 44.5%.
CONCLUSION(S): Preimplantation genetic diagnosis aneuploidy screening has a beneficial effect on pregnancy outcome in RM couples, especially those in which the woman is aged > or =35 years. Our data indicate that PGD reduces the risk of miscarriage in RM patients to baseline levels.
确定植入前基因诊断（PGD）及整倍体胚胎移植是否会降低复发性流产（RM）患者的自然流产率。
对照临床研究。
体外受精中心及PGD参考实验室。
有三次或更多次既往不明原因妊娠丢失的复发性流产患者。
对每个第3天胚胎的单个卵裂球进行活检，随后进行荧光原位杂交分析。
将接受PGD的RM受试者的自然流产率与[1]他们自己预先的预期以及[2]因高龄产妇（≥35岁）接受PGD的女性对照组进行比较。
在进行PGD之前，RM患者有87%（262/301）的妊娠丢失，预期丢失率为36.5%。之后，他们仅有16.7%的妊娠丢失。这种差异主要是由于≥35岁年龄亚组的妊娠丢失减少，从预期的44.5%降至12%。
植入前基因诊断非整倍体筛查对RM夫妇的妊娠结局有有益影响，尤其是女方年龄≥35岁的夫妇。我们的数据表明，PGD将RM患者的流产风险降低至基线水平。
