Azuma M, David L L, Shearer T R
Department of Biochemistry, School of Medicine, Oregon Health Sciences University, Portland 97201.
Ophthalmic Res. 1992;24(1):8-14. doi: 10.1159/000267138.
The purpose of this experiment was to determine the contribution of calpain proteolytic enzyme (EC 3.4.22.17) in the formation of nuclear cataract during lens culture in xylose. Increased lens calcium was found to be required for formation of xylose nuclear cataract in our culture system. Inhibition of calpain by the cysteine protease inhibitor E64 was effective in slowing the formation of nuclear cataract, even though lens calcium and hydration were markedly elevated. These results showed that hydration and elevated calcium alone do not produce xylose nuclear cataract, and they indicated that calpain proteolysis may be necessary for xylose nuclear cataract in the rat lens.
本实验的目的是确定钙蛋白酶（EC 3.4.22.17）在木糖晶状体培养过程中核性白内障形成中的作用。在我们的培养系统中发现，木糖核性白内障的形成需要晶状体钙含量增加。半胱氨酸蛋白酶抑制剂E64对钙蛋白酶的抑制作用有效地减缓了核性白内障的形成，尽管晶状体钙含量和水合作用显著升高。这些结果表明，单独的水合作用和钙含量升高并不会导致木糖核性白内障，它们表明钙蛋白酶的蛋白水解作用可能是大鼠晶状体中木糖核性白内障形成所必需的。
Zotero 插件