抗内皮细胞抗体（AECA）与丙硫氧嘧啶诱导的抗中性粒细胞胞浆抗体（ANCA）阳性血管炎的疾病活动相关。
[Anti-endothelial cell antibodies(AECA) are associated with disease activity of propylthiouracil induced antineutrophil cytoplasmic antibody (ANCA) positive vasculitis].
作者信息
Yu Feng, Zhao Ming-hui, Zhang Ying, Zhang You-kang
机构信息
Department of Nephrology, Peking University First Hospital, Beijing 100034, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2005 Aug 18;37(4):374-7.
OBJECTIVE
To investigate the prevalence of anti-endothelial cell antibodies(AECA) and its possible role in the pathogenesis of propylthiouracil (PTU) induced ANCA positive vasculitis.
METHODS
Sera from 11 patients with PTU induced ANCA positive vasculitis and 10 patients with PTU induced ANCA but without clinical vasculitis were studied. Soluble proteins from in vitro cultured human umbilical vein endothelial cells were used as antigens and immunoblotting technique was performed to identify the specific target antigens.
RESULTS
In patients with PTU induced ANCA positive vasculitis group, 10 of the 11 patients in active phase were AECA positive and 7 of the 10 patients turned to negative in remission. AECA consisted of a group of heterogeneous antibodies. In patients with ANCA positive but without vasculitis, none was AECA positive.
CONCLUSION
AECAs recognizing a variety of antigens could be found in sera from patients with PTU induced ANCA positive vasculitis and they had a much closer association with vasculitic disease activity compared with ANCA.
目的
探讨抗内皮细胞抗体（AECA）的患病率及其在丙硫氧嘧啶（PTU）诱导的抗中性粒细胞胞浆抗体（ANCA）阳性血管炎发病机制中的可能作用。
方法
研究11例PTU诱导的ANCA阳性血管炎患者和10例PTU诱导的ANCA阳性但无临床血管炎患者的血清。以体外培养的人脐静脉内皮细胞的可溶性蛋白作为抗原，采用免疫印迹技术鉴定特异性靶抗原。
结果
在PTU诱导的ANCA阳性血管炎组患者中，11例活动期患者中有10例AECA阳性，10例缓解期患者中有7例转为阴性。AECA由一组异质性抗体组成。在ANCA阳性但无血管炎的患者中，无AECA阳性。
结论
PTU诱导的ANCA阳性血管炎患者血清中可发现识别多种抗原的AECA，与ANCA相比，它们与血管炎疾病活动的相关性更强。
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