Berdel Wolfgang E, Kulimova Emma, Kolkmeyer Astrid, Zühlsdorf Michael, Serve Hubert, Büchner Thomas, Oelmann Elisabeth
Department of Medicine, Haematology and Oncology, University Hospital Muenster, 33 Albert-Schweitzer Street, 48149, Muenster, Germany.
Ann Hematol. 2005 Nov;84(12):771-3. doi: 10.1007/s00277-005-1088-3. Epub 2005 Nov 12.
Flow cytometry was applied to test for platelet-activating-factor receptor (PAF-R) presence on the membranes of acute myeloid leukemia (AML) cells. We have used six human AML cell lines and freshly taken density gradient separated blasts from the bone marrow of ten AML patients covering the majority of French-American-British (FAB) subtypes. Additionally, we have used one histiocytic lymphoma cell line and mature human granulocytes/monocytes as controls. Our results indicate lack of membrane PAF-R on AML of all FAB subtypes tested. This was particularly true for the more mature and differentiated subtypes M4 and M5, including monocytic cell elements, and the promyelocytic M3 AML. In contrast, membrane PAF-R could be easily detected in a histiocytic lymphoma cell line and mature granulocytes/monocytes from peripheral blood used as positive controls. In conclusion, this observation precludes the use of membrane PAF-R as an immunophenotypic marker for AML classification or detection of minimal residual disease.
应用流式细胞术检测急性髓系白血病（AML）细胞膜上血小板活化因子受体（PAF-R）的存在情况。我们使用了六种人类AML细胞系以及从十名AML患者骨髓中新鲜获取的经密度梯度分离的原始细胞，这些患者涵盖了大多数法美英（FAB）亚型。此外，我们还使用了一种组织细胞淋巴瘤细胞系以及成熟的人类粒细胞/单核细胞作为对照。我们的结果表明，在所检测的所有FAB亚型的AML中均不存在膜PAF-R。对于更成熟和分化的亚型M4和M5（包括单核细胞成分）以及早幼粒细胞M3 AML而言，情况尤其如此。相比之下，在用作阳性对照的组织细胞淋巴瘤细胞系以及外周血中的成熟粒细胞/单核细胞中可以很容易地检测到膜PAF-R。总之，这一观察结果排除了将膜PAF-R用作AML分类或检测微小残留病的免疫表型标志物的可能性。
