自然花粉暴露对季节性变应性鼻炎患者嗜酸性粒细胞凋亡的影响及其与支气管高反应性的关系。

The effect of natural pollen exposure on eosinophil apoptosis and its relationship to bronchial hyperresponsiveness in patients with seasonal allergic rhinitis.

作者信息

Kurt Emel, Bavbek Sevim, Aksu Ozhan, Erekul Selim, Misirligil Zeynep

机构信息

Department of Allergic Diseases, Ankara University, Medical Faculty, Ankara, Turkey.

出版信息

Ann Allergy Asthma Immunol. 2005 Jul;95(1):72-8. doi: 10.1016/S1081-1206(10)61191-X.

DOI:10.1016/S1081-1206(10)61191-X

PMID:16095145

Abstract

BACKGROUND

Limited data suggest that there is increased eosinophilic inflammation in the airways of patients with seasonal allergic rhinitis (SAR) during pollen season even if they do not have asthma.

OBJECTIVE

To investigate the effect of natural pollen exposure on inflammatory cells and apoptosis of eosinophils and its association with bronchial hyperresponsiveness (BHR) during and out of pollen season in SAR patients sensitized to only grass pollens.

METHODS

Forty SAR patients and 10 patients with nonallergic rhinitis (NAR) from Ankara, Turkey, were recruited to participate in the study. Two induced sputum samples were taken from SAR patients during pollen season (May-June) and out of pollen season (November-January), but only 1 induced sputum sample was taken from NAR patients. Slides of induced sputum were evaluated by 2 cytologists with the use of light microscopy after cytocentrifuged and dyed with May-Grünwald-Giemsa stain. Induced sputum samples were sufficient for differential cell counts in 14 SAR and 7 NAR patients.

RESULTS

Eosinophil counts in SAR patients were statistically higher in pollen season (19.4% +/- 16.2%) compared with out of season (4.6% +/- 6.9%, P = .003) and with NAR patients (4.7% +/- 9.5%, P = .01). The apoptotic eosinophil counts in SAR patients were statistically higher out of pollen season (3.0% +/- 4.5%) than in pollen season (0.38% +/- 0.80%, P = .02) and higher than those of NAR patients (0.14% +/- 0.26%, P = .005). The apoptotic ratio was statistically higher after pollen season compared with pollen season (0.720% +/- 0.394% vs 0.044% +/- 0.116%, P = .002). Blood eosinophil counts of SAR patients were increased during the pollen season (364 +/- 187/mm3) compared with out of season (278 +/- 219/mm3, P = .04) and with NAR patients (85 +/- 54/mm3, P = .001). The number of SAR patients who had BHR during the pollen season (7/14) was higher than the number who had BHR out of season (2/14, chi2 = 4.2, P = .04).

CONCLUSION

Our data indicate that changes in eosinophil counts and eosinophil apoptosis may be related to the changes of natural pollen exposure and seasonal changes of BHR in SAR patients.

摘要

背景

有限的数据表明，即使没有哮喘，季节性变应性鼻炎（SAR）患者在花粉季节气道中的嗜酸性粒细胞炎症也会增加。

目的

研究天然花粉暴露对仅对草花粉致敏的SAR患者在花粉季节期间及非花粉季节炎症细胞和嗜酸性粒细胞凋亡的影响及其与支气管高反应性（BHR）的关系。

方法

招募了来自土耳其安卡拉的40例SAR患者和10例非变应性鼻炎（NAR）患者参与研究。在花粉季节（5月至6月）和非花粉季节（11月至1月）从SAR患者采集两份诱导痰样本，但仅从NAR患者采集一份诱导痰样本。诱导痰涂片经细胞离心并使用May-Grünwald-Giemsa染色后，由2名细胞学家通过光学显微镜进行评估。14例SAR患者和7例NAR患者的诱导痰样本足以进行细胞分类计数。

结果

与非花粉季节（4.6%±6.9%，P = 0.003）以及NAR患者（4.7%±9.5%，P = 0.01）相比，SAR患者在花粉季节的嗜酸性粒细胞计数在统计学上更高（19.4%±16.2%）。SAR患者在非花粉季节的凋亡嗜酸性粒细胞计数在统计学上高于花粉季节（3.0%±4.5%）（0.38%±0.80%，P = 0.02），且高于NAR患者（0.14%±0.26%，P = 0.005）。与花粉季节相比，花粉季节后凋亡率在统计学上更高（0.720%±0.394%对0.044%±0.116%，P = 0.002）。与非花粉季节（278±219/mm³，P = 0.04）以及NAR患者（85±54/mm³，P = 0.001）相比，SAR患者在花粉季节的外周血嗜酸性粒细胞计数增加（364±187/mm³）。花粉季节期间出现BHR的SAR患者数量（7/14）高于非花粉季节（2/14，χ² = 4.2，P = 0.04）。