Tzirogiannis Konstantinos N, Panoutsopoulos Georgios I, Papadimas George K, Demonakou Maria D, Hereti Rosa I, Manta Georgia A, Kourentzi Kalliopi T, Kondili Vasiliki G, Alexandropoulou Katerina N, Mykoniatis Michael G
Department of Experimental Pharmacology, Medical School, Athens University, 75 Mikras Asias Street, Athens 115 27, Greece.
In Vivo. 2005 Sep-Oct;19(5):841-7.
The restorative effect of hepatic stimulator substance (HSS) against hepatic regeneration arrest induced by 5-HT2 receptor blockade was investigated.
Male Wistar rats were subjected to 60-70% partial hepatectomy and to 5-HT2 receptor blockade at 16 h after partial hepatectomy by ketanserin administration (6 mg/kg bodyweight intraperitoneally; group I). HSS at the dose of 100 mg protein/kg bodyweight was administered at 10 or 17 h after partial hepatectomy in ketanserin-treated rats (groups II and III). The mitotic index in hematoxylin-eosin-stained liver sections, immunochemical detection of PCNA and Ki 67 nuclear antigens and the rate of [3H]-thymidine incorporation into hepatic DNA were used as indices of liver regeneration.
Liver regeneration, as evaluated by [3H]-thymidine incorporation into hepatic DNA, mitotic index, PCNA and Ki67 nuclear antigens, peaked at 40 h in groups I, II and III of rats and no significant differences were observed between the studied groups.
HSS administration is not capable of reversing the liver regeneration arrest induced by 5-HT2 receptor blockade.
研究了肝刺激物质（HSS）对5-羟色胺2（5-HT2）受体阻断诱导的肝再生停滞的恢复作用。
雄性Wistar大鼠接受60 - 70%的部分肝切除术，并在部分肝切除术后16小时通过给予酮色林（6毫克/千克体重，腹腔注射；第一组）进行5-HT2受体阻断。在酮色林处理的大鼠中，于部分肝切除术后10或17小时给予剂量为100毫克蛋白质/千克体重的HSS（第二组和第三组）。苏木精-伊红染色肝切片中的有丝分裂指数、PCNA和Ki 67核抗原的免疫化学检测以及[3H]-胸腺嘧啶核苷掺入肝DNA的速率被用作肝再生的指标。
通过[3H]-胸腺嘧啶核苷掺入肝DNA、有丝分裂指数、PCNA和Ki67核抗原评估的肝再生，在第一、二、三组大鼠中于40小时达到峰值，且研究组之间未观察到显著差异。
给予HSS不能逆转5-HT2受体阻断诱导的肝再生停滞。
