肝刺激物质（HSS）对5-HT2受体阻断诱导的肝再生停滞的影响。

The effect of hepatic stimulator substance (HSS) on liver regeneration arrest induced by 5-HT2 receptor blockade.

作者信息

Tzirogiannis Konstantinos N, Panoutsopoulos Georgios I, Papadimas George K, Demonakou Maria D, Hereti Rosa I, Manta Georgia A, Kourentzi Kalliopi T, Kondili Vasiliki G, Alexandropoulou Katerina N, Mykoniatis Michael G

机构信息

Department of Experimental Pharmacology, Medical School, Athens University, 75 Mikras Asias Street, Athens 115 27, Greece.

出版信息

In Vivo. 2005 Sep-Oct;19(5):841-7.

PMID:16097436

Abstract

BACKGROUND

The restorative effect of hepatic stimulator substance (HSS) against hepatic regeneration arrest induced by 5-HT2 receptor blockade was investigated.

MATERIALS AND METHODS

Male Wistar rats were subjected to 60-70% partial hepatectomy and to 5-HT2 receptor blockade at 16 h after partial hepatectomy by ketanserin administration (6 mg/kg bodyweight intraperitoneally; group I). HSS at the dose of 100 mg protein/kg bodyweight was administered at 10 or 17 h after partial hepatectomy in ketanserin-treated rats (groups II and III). The mitotic index in hematoxylin-eosin-stained liver sections, immunochemical detection of PCNA and Ki 67 nuclear antigens and the rate of [3H]-thymidine incorporation into hepatic DNA were used as indices of liver regeneration.

RESULTS

Liver regeneration, as evaluated by [3H]-thymidine incorporation into hepatic DNA, mitotic index, PCNA and Ki67 nuclear antigens, peaked at 40 h in groups I, II and III of rats and no significant differences were observed between the studied groups.

CONCLUSION

HSS administration is not capable of reversing the liver regeneration arrest induced by 5-HT2 receptor blockade.

摘要

背景

研究了肝刺激物质（HSS）对5-羟色胺2（5-HT2）受体阻断诱导的肝再生停滞的恢复作用。

材料与方法

雄性Wistar大鼠接受60 - 70%的部分肝切除术，并在部分肝切除术后16小时通过给予酮色林（6毫克/千克体重，腹腔注射；第一组）进行5-HT2受体阻断。在酮色林处理的大鼠中，于部分肝切除术后10或17小时给予剂量为100毫克蛋白质/千克体重的HSS（第二组和第三组）。苏木精-伊红染色肝切片中的有丝分裂指数、PCNA和Ki 67核抗原的免疫化学检测以及[3H]-胸腺嘧啶核苷掺入肝DNA的速率被用作肝再生的指标。