Ozcakir Hasan Tayfun, Giray Seren Gulsen, Ozbilgin Mahmut Kemal, Uyar Yýldýz, Lacin Selman, Caglar Husnu
Department of Obstetrics and Gynecology, Celal Bayar University Hospital, Manisa, Turkey.
Acta Obstet Gynecol Scand. 2005 Sep;84(9):887-93. doi: 10.1111/j.0001-6349.2005.00586.x.
The aim of the present study is to figure out the immunohistochemical expression of transforming growth factor-alpha (TGF-alpha), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) in hyperstimulated rat ovaries.
Twenty Wistar-Albino adult female rats (250-300 g) were taken into the study. The animals were randomly divided into two groups, each containing 10 rats: (i) stimulation group and (ii) control group. In the stimulation group, a stimulation regimen was administered to induce follicular maturity and ovarian hyperstimulation syndrome (OHSS) at the end using a 30-IU follicle-stimulating hormone that was administered subcutaneously for 4 consecutive days, followed by a 30-IU human chorionic gonadotropin on day 5 to induce ovulation. The rats, in the control group, received 0.2 ml of 0.9% NaCl for 5 consecutive days to mimic the conditions of the study animals. At the end of the treatment period, all rats underwent ovariectomy and the sections of ovaries were stained for the TGF-alpha, EGF, and VEGF.
The expression of TGF-alpha, EGF, and VEGF in the endothelium, the stroma, the granulosa cells, and the corpus luteum was found to be significantly higher in the stimulated group, compared to that in the control group ( p < 0.05).
TGF-alpha, EGF, and VEGF are found to have increased in the hyperstimulated ovaries and this finding seems to be involved in the OHSS pathogenesis.
本研究旨在明确转化生长因子-α（TGF-α）、表皮生长因子（EGF）和血管内皮生长因子（VEGF）在超刺激大鼠卵巢中的免疫组化表达情况。
选取20只成年Wistar白化雌性大鼠（体重250 - 300克）纳入研究。将动物随机分为两组，每组10只：（i）刺激组和（ii）对照组。在刺激组中，采用刺激方案诱导卵泡成熟并最终诱发卵巢过度刺激综合征（OHSS），即连续4天皮下注射30国际单位的促卵泡激素，随后在第5天注射30国际单位的人绒毛膜促性腺激素以诱导排卵。对照组大鼠连续5天接受0.2毫升0.9%氯化钠溶液注射，以模拟研究动物的情况。在治疗期结束时，所有大鼠均接受卵巢切除术，并对卵巢切片进行TGF-α、EGF和VEGF染色。
与对照组相比，刺激组内皮、基质、颗粒细胞和黄体中TGF-α、EGF和VEGF的表达显著更高（p < 0.05）。
发现超刺激卵巢中TGF-α、EGF和VEGF增加，这一发现似乎与OHSS的发病机制有关。
