Ohkawa Ryunosuke, Hirowatari Yuji, Nakamura Kazuhiro, Ohkubo Shigeo, Ikeda Hitoshi, Okada Mitsumasa, Tozuka Minoru, Nakahara Kazuhiko, Yatomi Yutaka
Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
Clin Biochem. 2005 Nov;38(11):1023-6. doi: 10.1016/j.clinbiochem.2005.07.008. Epub 2005 Aug 10.
Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated.
Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method.
Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin.
Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
评估血浆样本中α颗粒衍生的CXC趋化因子和致密颗粒衍生的血清素的血小板释放情况。
采用酶免疫测定法检测CXC趋化因子β-血小板球蛋白（β-TG）和血小板第4因子（PF4）的浓度；采用高效液相色谱法测定血清素。
体外操作时，β-TG和PF4比血清素更容易释放。使用抗血小板混合剂枸橼酸钠-茶碱-腺苷-双嘧达莫（CTAD）并将样本保存在4℃对于准确测量β-TG和PF4是必要的，但对血清素测量则不然。
由于血浆样本制备简便，检测血清素作为一项实验室检测可能有助于评估体内血小板活化情况。
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