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凡纳滨对虾重组溶菌酶对革兰氏阴性菌具有抗菌活性，这些革兰氏阴性菌包括：溶藻弧菌、副溶血性弧菌和霍乱弧菌。

White shrimp (Litopenaeus vannamei) recombinant lysozyme has antibacterial activity against Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae.

作者信息

de-la-Re-Vega Enrique, García-Galaz Alfonso, Díaz-Cinco Martha E, Sotelo-Mundo Rogerio R

机构信息

Aquatic Molecular Biology Laboratory, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora 83000, Mexico.

出版信息

Fish Shellfish Immunol. 2006 Mar;20(3):405-8. doi: 10.1016/j.fsi.2005.06.005.

DOI:10.1016/j.fsi.2005.06.005
PMID:16098764
Abstract

C-type lysozyme has been described as an antibacterial component of the shrimp innate defence system. We determined quantitatively the antibacterial activity of white shrimp (Litopenaeus vannamei) recombinant lysozyme against three Gram negative bacteria: Vibrio alginolyticus, Vibrio parahemolyticus and Vibrio cholerae, using a turbidimetric assay with live bacteria and differential bacterial viable count after interaction with the protein. In conclusion, the antibacterial activity of recombinant shrimp lysozyme against Vibrio sp. is at least equal to the values against the Gram positive M. luteus and more active against the shrimp pathogens V. alginolyticus and V. parahemolyticus.

摘要

C型溶菌酶已被描述为虾先天防御系统的一种抗菌成分。我们使用活菌比浊法以及与该蛋白相互作用后的细菌活菌计数差异，定量测定了凡纳滨对虾重组溶菌酶对三种革兰氏阴性菌的抗菌活性，这三种革兰氏阴性菌分别是：溶藻弧菌、副溶血性弧菌和霍乱弧菌。总之，重组虾溶菌酶对弧菌属的抗菌活性至少等同于对革兰氏阳性菌藤黄微球菌的抗菌活性值，且对虾病原体溶藻弧菌和副溶血性弧菌的抗菌活性更强。

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