荧光染色在诊断合并感染HIV的肺结核中的效能

Efficacy of fluorochrome stain in the diagnosis of pulmonary tuberculosis co-infected with HIV.

作者信息

Prasanthi K, Kumari A R

机构信息

Department of Microbiology, Gandhi Medical College, Musheerabad, Secunderabad-500 025, Andhra Pradesh, India.

出版信息

Indian J Med Microbiol. 2005 Jul;23(3):179-81. doi: 10.4103/0255-0857.16591.

DOI:10.4103/0255-0857.16591

PMID:16100425

Abstract

UNLABELLED

The resurgence of Mycobacterium tuberculosis in association with HIV infection has focussed much attention in the rapid diagnosis of high risk cases. Infection with HIV is known to alter the presentation of pulmonary tuberculosis. The present study was under taken to compare the efficacy of fluorochrome stain (Fl) with conventional Ziehl Neelsen (ZN) stain in the diagnosis of pulmonary tuberculosis. Two hundred cases of pulmonary tuberculosis were included in the study. Sputum smears were screened for acid fast bacilli (AFB) by ZN and Fl methods and blood samples were screened for HIV. Sputum positive cases detected by Fl stain were higher in number (69%) when compared to ZN stain (50%). Of the total cases studied 15.5% were HIV seropositive.

CONCLUSIONS

Fluorochrome staining was found to be more efficient (45%) when compared to ZN staining (29%) in detecting cases associated with HIV seropositivity, especially paucibacillary cases.

摘要

未标记

结核分枝杆菌与HIV感染相关的再度流行已使人们高度关注高危病例的快速诊断。已知HIV感染会改变肺结核的表现形式。本研究旨在比较荧光染色（Fl）与传统齐-尼（ZN）染色在肺结核诊断中的效果。该研究纳入了200例肺结核病例。通过ZN法和Fl法对痰涂片进行抗酸杆菌（AFB）筛查，并对血样进行HIV筛查。与ZN染色（50%）相比，Fl染色检测出的痰阳性病例数量更多（69%）。在所有研究病例中，15.5%为HIV血清阳性。