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Preliminary x-ray crystallographic analysis of centrin from ciliate Euplotes octocarinatus.

作者信息

He Xiao-Jing, Li Liu, Hou Hai, Zhao Yaqin, Liang Aihua, Rao Zihe

机构信息

Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China.

出版信息

Protein Pept Lett. 2005 Aug;12(6):609-11. doi: 10.2174/0929866054395842.

Abstract

Centrins are four-EF-hand Ca(2+)-binding proteins, which belong to the CaM super family. The centrin from ciliate Euplotes Octocarinatus has been expressed in Escherichia coli, purified and crystallized using the hanging-drop method. Rod-like crystals were grown and diffracted to 2.0 angstroms. The crystals belong to space group P2(1)2(1)2(1) and the unit-cell parameters are a=34.442 angstroms, b=48.954 angstroms, c=72.583 angstroms.

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