一种适用于水稻中小规模DNA提取的叶片组织快速简易匀浆方法。

Rapid and simple procedure for homogenizing leaf tissues suitable for mini-midi-scale DNA extraction in rice.

作者信息

Yi Gihwan, Choi Jun-Ho, Lee Jong-Hee, Jeong Unggi, Nam Min-Hee, Yun Doh-Won, Eun Moo-Young

机构信息

Rice Division, Yeongnam Agricultural Research Institute, Milyang, Korea.

出版信息

Prep Biochem Biotechnol. 2005;35(3):257-61. doi: 10.1081/PB-200065652.

DOI:10.1081/PB-200065652

PMID:16109637

Abstract

We describe a rapid and simple procedure for homogenizing leaf samples suitable for mini/midi-scale DNA preparation in rice. The methods used tungsten carbide beads and general vortexer for homogenizing leaf samples. In general, two samples can be ground completely within 11.3+/-1.5 sec at one time. Up to 20 samples can be ground at a time using a vortexer attachment. The yields of the DNA ranged from 2.2 to 7.6 microg from 25-150 mg of young fresh leaf tissue. The quality and quantity of DNA was compatible for most of PCR work and RFLP analysis.

摘要

我们描述了一种快速简便的方法，用于将水稻叶片样品匀浆，以适用于小规模/中规模DNA制备。该方法使用碳化钨珠和普通涡旋仪来匀浆叶片样品。一般来说，一次可在11.3±1.5秒内将两个样品完全研磨。使用涡旋仪附件一次最多可研磨20个样品。从25 - 150毫克新鲜幼嫩叶片组织中提取的DNA产量在2.2至7.6微克之间。所提取DNA的质量和数量适用于大多数PCR工作和RFLP分析。