沙眼衣原体感染的Hela229细胞诱导的SOCS-1、3的表达
[Expression of SOCS-1, 3 induced by Chlamydia trachomatis-infected Hela229].
作者信息
Wei Xiu-qing, Cheng Wen, Yan Jie, Mei Bing, Huo Zhi, Yu Ping
机构信息
Department of Immunology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Dec;29(6):639-42.
OBJECTIVE
To explore the relationship between SOCS, immune suppression and chlamydial persistence of Chlamydia trachomatis-infected cells.
METHODS
After being infected with Chlamydia trachomatis, RT-PCR was used to examine the expression of SOCS-1, 3 mRNA and Western blotting to examine the expression of SOCS-1,3 protein in Hela229 cells. Immunofluorescence was used to examine the effect of SOCS obstruction on the multiplication of Chlamydia trachomatis.
RESULTS
Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3 both on mRNA level and on protein level. The multiplicity of Chlamydia trchomatis can be suppressed after the expression of SOCS-1,3 was obstructed.
CONCLUSION
Chlamydia trachomatis-infected Hela229 cells can induce the expression of SOCS-1,3, which is one of the mechanisms that Chlamydia trachomatis can suppress host immune and result in persistence.
目的
探讨细胞因子信号转导抑制因子（SOCS）、免疫抑制与沙眼衣原体感染细胞中衣原体持续存在之间的关系。
方法
用沙眼衣原体感染Hela229细胞后，采用逆转录-聚合酶链反应（RT-PCR）检测细胞中细胞因子信号转导抑制因子1（SOCS-1）、3 mRNA的表达，蛋白质免疫印迹法检测SOCS-1、3蛋白的表达。采用免疫荧光法检测SOCS阻断对沙眼衣原体增殖的影响。
结果
沙眼衣原体感染的Hela229细胞可在mRNA水平和蛋白水平诱导SOCS-1、3的表达。阻断SOCS-1、3的表达后，沙眼衣原体的增殖能力受到抑制。
结论
沙眼衣原体感染的Hela229细胞可诱导SOCS-1、3的表达，这是沙眼衣原体抑制宿主免疫并导致持续感染的机制之一。
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