Nigútová Katarína, Pristas Peter, Javorský Peter
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovak Republic.
Arch Anim Nutr. 2005 Jun;59(3):205-11. doi: 10.1080/17450390500148121.
Antimicrobial proteins and peptides produced by bacteria, termed bacteriocins, are widely acknowledged to be important contributors to their producer organism survival. Enterocin A, enterocin B, enterocin P and enterolysin A belong to the best studied enterocins, i.e., bacteriocins produced by enterococci and streptococci. Twenty-one enterococcal and seven streptococcal isolates were analysed for bacteriocin-like activity production and resistance by overlay test. Up to 50% of tested strains showed antibacterial activity at least against one indicator strain. The occurrence of enterocin B structural gene in several isolates was confirmed by PCR method. The results of this study should broaden knowledge of bacteriocin-like activity production and resistance among gram-positive bacteria.
细菌产生的抗菌蛋白和肽,即细菌素,被广泛认为是其产生菌生存的重要因素。肠球菌A、肠球菌B、肠球菌P和肠球菌溶血素A属于研究得最为深入的肠球菌素,即由肠球菌和链球菌产生的细菌素。通过覆盖试验分析了21株肠球菌和7株链球菌分离株的细菌素样活性产生情况及抗性。高达50%的受试菌株至少对一种指示菌株表现出抗菌活性。通过PCR方法证实了几种分离株中存在肠球菌B结构基因。本研究结果应能拓宽革兰氏阳性菌中细菌素样活性产生及抗性方面的知识。