开放启动子复合物内稳定的DNA解旋由RNA聚合酶β' - 钳域介导。
Stable DNA opening within open promoter complexes is mediated by the RNA polymerase beta'-jaw domain.
作者信息
Wigneshweraraj Siva R, Burrows Patricia C, Severinov Konstantin, Buck Martin
机构信息
Division of Biology, Faculty of Life Sciences, Sir Alexander Fleming Building, Imperial College London, London SW7 2AZ, United Kingdom.
出版信息
J Biol Chem. 2005 Oct 28;280(43):36176-84. doi: 10.1074/jbc.M506416200. Epub 2005 Aug 24.
DNA opening for transcription-competent open promoter complex (OC) formation by the bacterial RNA polymerase (RNAP) relies upon a complex network of interactions between the structurally conserved and flexible modules of the catalytic beta and beta'-subunits, RNAP-associated sigma-subunit, and the DNA. Here, we show that one such module, the beta'-jaw, functions to stabilize the OC. In OCs formed by the major sigma70-RNAP, the stabilizing role of the beta'-jaw is not restricted to any particular melted DNA segment. In contrast, in OCs formed by the major variant sigma54-RNAP, the beta'-jaw and a conserved sigma54 regulatory domain co-operate to stabilize the melted DNA segment immediately upstream of the transcription start site. Clearly, regulated communication between the mobile modules of the RNAP and the functional domain(s) of the sigma subunit is required for stable DNA opening.
细菌RNA聚合酶（RNAP）形成具有转录活性的开放启动子复合物（OC）时，DNA解旋依赖于催化性β亚基和β'亚基、与RNAP相关的σ亚基以及DNA的结构保守和灵活模块之间复杂的相互作用网络。在此，我们表明，其中一个这样的模块，即β'颚，起到稳定OC的作用。在由主要的σ70-RNAP形成的OC中，β'颚的稳定作用并不局限于任何特定的解旋DNA片段。相比之下，在由主要变体σ54-RNAP形成的OC中，β'颚和一个保守的σ54调节结构域协同作用，以稳定转录起始位点上游紧邻的解旋DNA片段。显然，RNAP的可移动模块与σ亚基的功能结构域之间的调控通讯是稳定DNA解旋所必需的。
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