乙型肝炎病毒X蛋白与异三聚体鸟嘌呤核苷酸结合蛋白的β5亚基相互作用。

Hepatitis B virus X protein interacts with beta5 subunit of heterotrimeric guanine nucleotide binding protein.

作者信息

Lwa Siew Hui, Chen Wei Ning

机构信息

School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Blk 1 Innovation Centre, 637722, Singapore.

出版信息

Virol J. 2005 Aug 31;2:76. doi: 10.1186/1743-422X-2-76.

DOI:10.1186/1743-422X-2-76

PMID:16135253

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1215529/

Abstract

BACKGROUND

To isolate cellular proteins interacting with hepatitis B virus X protein (HBX), from HepG2 cells infected with hepatitis B virus (HBV).

RESULTS

HBV particles were produced in culture medium of HepG2 cells transfected with the mammalian expression vector containing the linear HBV genome, as assessed by commercially available ELISA assay. A cDNA library was made from these cells exposed to HBV. From yeast two hybrid screening with HBX as bait, human guanine nucleotide binding protein beta subunit 5L (GNbeta5) was isolated from the cDNA library constructed in this study as a new HBX-interacting protein. The HBX-GNbeta5 interaction was further supported by mammalian two hybrid assay.

CONCLUSION

The use of a cDNA library constructed from HBV-transfected HepG2 cells has resulted in the isolation of new cellular proteins interacting with HBX.

摘要

背景

从感染乙型肝炎病毒（HBV）的HepG2细胞中分离与乙型肝炎病毒X蛋白（HBX）相互作用的细胞蛋白。

结果

通过市售ELISA检测评估，转染了含线性HBV基因组的哺乳动物表达载体的HepG2细胞培养基中产生了HBV颗粒。从暴露于HBV的这些细胞构建了一个cDNA文库。以HBX为诱饵进行酵母双杂交筛选，从本研究构建的cDNA文库中分离出人类鸟嘌呤核苷酸结合蛋白β亚基5L（GNbeta5）作为一种新的与HBX相互作用的蛋白。哺乳动物双杂交试验进一步证实了HBX与GNbeta5的相互作用。