大肠杆菌中ArcA/ArcB双组分系统对DNA修复基因（uvrA）表达的负调控

Negative regulation of DNA repair gene (uvrA) expression by ArcA/ArcB two-component system in Escherichia coli.

作者信息

Ogasawara Hiroshi, Teramoto Jun, Yamamoto Satoshi, Hirao Kiyo, Yamamoto Kaneyoshi, Ishihama Akira, Utsumi Ryutaro

机构信息

Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan.

出版信息

FEMS Microbiol Lett. 2005 Oct 15;251(2):243-9. doi: 10.1016/j.femsle.2005.08.006.

DOI:10.1016/j.femsle.2005.08.006

PMID:16140472

Abstract

The ArcA/ArcB two-component signal transduction system of Escherichia coli regulates gene expression in response to the redox conditions of growth. In this study, uvrA gene expression was repressed when ArcA was induced in E. coli. Transcription of uvrA increased in DeltaarcA and DeltaarcB strains more than in the wild-type strain, whose trend was remarkable under the anaerobic condition. In the wild-type strain grown in the presence of DTT (10 mM), the uvrA gene expression was also repressed. Furthermore, the results of in vitro transcription and DNase I footprinting experiments indicated that ArcA specifically bound to the ArcA box [(A/T)GTTAATTA(A/T)] in the uvrA promoter and represses its transcription. These results suggest that the ArcA/ArcB two-component system works to negatively regulate uvrA gene expression.

摘要

大肠杆菌的ArcA/ArcB双组分信号转导系统根据生长的氧化还原条件调节基因表达。在本研究中，当在大肠杆菌中诱导ArcA时，uvrA基因表达受到抑制。uvrA的转录在ΔarcA和ΔarcB菌株中比野生型菌株中增加得更多，在厌氧条件下这种趋势很明显。在存在二硫苏糖醇（10 mM）的情况下生长的野生型菌株中，uvrA基因表达也受到抑制。此外，体外转录和DNase I足迹实验结果表明，ArcA特异性结合uvrA启动子中的ArcA框[（A/T）GTTAATTA（A/T）]并抑制其转录。这些结果表明，ArcA/ArcB双组分系统对uvrA基因表达起负调控作用。