Li Jian-Sen, Chen Fa-Xian, Shikiya Ronald, Marky Luis A, Gold Barry
Eppley Institute for Research in Cancer and Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Nebraska Medical Center, Omaha, Nebraska 68198-6805, USA.
J Am Chem Soc. 2005 Sep 14;127(36):12657-65. doi: 10.1021/ja0530218.
Stable DNA triple-helical structures are normally restricted to homopurine sequences. We have described a system of four heterocyclic bases (TRIPsides) that, when incorporated into oligomers (oligoTRIPs), can recognize and bind in the major groove to any native sequence of DNA [Li et al., J. Am. Chem. Soc. 2003, 125, 2084]. To date, we have reported on triplex-forming oligomers composed of two of these TRIPsides, i.e., antiTA and antiGC, and their ability to form intramolecular triplexes at mixed purine/pyrimidine sequences. In the present study, we describe the synthesis and characterization of the antiCG TRIPside and its use in conjunction with antiTA and antiGC to form sequence-specific intra- and/or intermolecular triplex structures at mixed purine/pyrimidine sequences that require as many as four major groove crossovers.
稳定的DNA三螺旋结构通常局限于同型嘌呤序列。我们已经描述了一种由四个杂环碱基组成的系统(TRIPsides),当将其掺入寡聚物(oligoTRIPs)中时,能够在大沟中识别并结合到任何天然DNA序列上[Li等人,《美国化学会志》2003年,125卷,2084页]。迄今为止,我们已经报道了由其中两个TRIPsides,即antiTA和antiGC组成的三链形成寡聚物,以及它们在嘌呤/嘧啶混合序列处形成分子内三链体的能力。在本研究中,我们描述了antiCG TRIPside的合成与表征,以及它与antiTA和antiGC结合使用,在需要多达四个大沟交叉的嘌呤/嘧啶混合序列处形成序列特异性的分子内和/或分子间三链体结构的情况。