Aufderheide Adam C, Athanasiou Kyriacos A
Department of Bioengineering, Rice University, Houston, TX 77251, USA.
Tissue Eng. 2005 Jul-Aug;11(7-8):1095-104. doi: 10.1089/ten.2005.11.1095.
The menisci of the knee are semilunar fibrocartilaginous structures critical in load bearing, shock absorption, stability, and lubrication. In this study, two commonly used biomaterials, a hydrogel (agarose) and a nonwoven mesh polymer [poly(glycolic acid); PGA], were compared for suitability as scaffold materials for tissue engineering the knee meniscus. In addition, a rotating wall bioreactor culture of both scaffold materials was compared with static cultures. Constructs were cultured for up to 7 weeks in static and rotating wall bioreactor culture. Cell numbers were 22 times higher in PGA than agarose after 7 weeks in culture. Static PGA scaffolds had more than twice the amount of sulfated glycosaminoglycans and three times the amount of collagen compared to static agarose constructs at week 7. The rotating wall bioreactor was not found with increase matrix production or cell proliferation significantly over static cultures.
膝关节半月板是半月形纤维软骨结构，在承重、减震、稳定和润滑方面起着关键作用。在本研究中，比较了两种常用生物材料——水凝胶（琼脂糖）和非织造网状聚合物[聚乙醇酸（PGA）]作为膝关节半月板组织工程支架材料的适用性。此外，还比较了两种支架材料在旋转壁生物反应器中的培养与静态培养。构建物在静态和旋转壁生物反应器培养中培养长达7周。培养7周后，PGA中的细胞数量比琼脂糖中的高22倍。在第7周时，静态PGA支架中的硫酸化糖胺聚糖含量是静态琼脂糖构建物的两倍多，胶原蛋白含量是其三倍。未发现旋转壁生物反应器与静态培养相比能显著增加基质产生或细胞增殖。
