McCarthy Joan M, Capullari Tammy, Spellacy William N
Department of Obstetrics and Gynecology, University of South Florida College of Medicine, Tampa, FL 33606, USA.
J Matern Fetal Neonatal Med. 2005 Mar;17(3):199-201. doi: 10.1080/14767050500073175.
To determine the correlation between automated hematology nucleated red blood cell counts and manual counts in umbilical cord blood.
Umbilical cord venous blood was obtained after cord clamping at the time of delivery. The number of nucleated red blood cells per one hundred white blood cells was measured using an automated hematology analyzer and compared with direct microscopic visualization of prepared slides by experienced hematology technicians.
The umbilical cord blood from 128 women was studied. The mean, median and standard deviations of nucleated red blood cell counts were not significantly different between the automated reading and the manual reading (mean 9.3+/-11.2 versus 9.1+/-13.1, respectively, P=0.76; median 5.9 versus 5.0, P=0.95; range 0-77, 0-105). Automated derived nucleated red blood cell counts and manual numbers were highly correlated with a correlation coefficient of 0.794.
The automated hematology analyzer readings of nucleated red blood cell counts correlate well with readings by laboratory hematologists.
确定脐血中自动血液学有核红细胞计数与手工计数之间的相关性。
分娩时脐带钳夹后采集脐静脉血。使用自动血液分析仪测量每100个白细胞中有核红细胞的数量,并与经验丰富的血液学技术人员对制备玻片的直接显微镜观察结果进行比较。
对128名女性的脐血进行了研究。自动读数和手工读数之间有核红细胞计数的均值、中位数和标准差无显著差异(均值分别为9.3±11.2和9.1±13.1,P = 0.76;中位数分别为5.9和5.0,P = 0.95;范围为0 - 77,0 - 105)。自动得出的有核红细胞计数与手工计数高度相关,相关系数为0.794。
自动血液分析仪对有核红细胞计数的读数与实验室血液学家的读数相关性良好。