Baltaci Abdulkerim Kasim, Mogulkoc Rasim, Ozturk Ahmet
Selcuk University Meram Medical School Department of Physiology, 42080 Konya, Turkey.
Life Sci. 2006 Jan 11;78(7):746-52. doi: 10.1016/j.lfs.2005.05.098. Epub 2005 Sep 8.
The present study aims to examine how zinc and testosterone supplementation, in combination and separately, affect plasma LH, FSH and leptin levels in castrated rats. Eighty experimental animals used in the study were allocated to 8 groups, each containing an equal number of rats. Group 1, control group; Group 2, castration group; Group 3, testosterone group (5 mg/kg/day); Group 4, zinc-supplemented group (3 mg/kg/day); Group 5, testosterone and zinc-supplemented group; Group 6, zinc-supplemented castration group; Group 7, testosterone and castration group; and Group 8, zinc-supplemented, testosterone and castration group. Plasma zinc, leptin, LH, FSH and free and total testosterone levels were determined in the blood samples collected from the animals by decapitation. Group 2 had the highest leptin levels and together with group 6, it also showed the highest LH and FSH levels (p<0.01). The lowest leptin levels were observed in groups 3 and 7 (p<0.01). Leptin levels in groups 4 and 6 were higher than those in groups 1, 5 and 8 (p<0.01). LH levels in group 4 were lower than those in groups 2 and 6 and higher than those in all other groups (p<0.01). Free and total testosterone levels in groups 7 and 8 were lower than those in groups 3 and 5, but higher than those in all other groups (p<0.01). Plasma LH levels may be more effective than testosterone on plasma leptin and zinc may be an important mediator of the effect LH has on leptin.
本研究旨在探讨锌和睾酮单独及联合补充对去势大鼠血浆促黄体生成素（LH）、促卵泡生成素（FSH）和瘦素水平的影响。本研究中使用的80只实验动物被分为8组，每组大鼠数量相等。第1组为对照组；第2组为去势组；第3组为睾酮组（5毫克/千克/天）；第4组为补锌组（3毫克/千克/天）；第5组为睾酮和锌联合补充组；第6组为补锌去势组；第7组为睾酮和去势组；第8组为补锌、睾酮和去势组。通过断头采集动物血液样本，测定血浆锌、瘦素、LH、FSH以及游离睾酮和总睾酮水平。第2组瘦素水平最高，与第6组一起，其LH和FSH水平也最高（p<0.01）。第3组和第7组瘦素水平最低（p<0.01）。第4组和第6组的瘦素水平高于第1组、第5组和第8组（p<0.01）。第4组的LH水平低于第2组和第6组，但高于所有其他组（p<0.01）。第7组和第8组的游离睾酮和总睾酮水平低于第3组和第5组，但高于所有其他组（p<0.01）。血浆LH水平对血浆瘦素的影响可能比睾酮更有效，锌可能是LH对瘦素产生影响的重要介质。
