乳腺癌中Her-2/neu的表达——不同诊断方法的比较
Her-2/neu expression in breast cancer--A comparison of different diagnostic methods.
作者信息
Benöhr P, Henkel V, Speer R, Vogel U, Sotlar K, Aydeniz B, Reiser A, Neubauer H, Tabiti K, Wallwiener D, Clare S E, Kurek R
机构信息
Department of Nephrology and Rheumatology, University of Göttingen, Germany.
出版信息
Anticancer Res. 2005 May-Jun;25(3B):1895-900.
BACKGROUND
Determination of Her-2/neu overexpression in breast cancer has previously been shown to be of prognostic significance. In this study, Her-2/neu expression in breast cancer was characterised by real-time PCR (RLT-PCR) based LightCycler-HER-2/neu DNA Quantification with immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH).
MATERIAL AND METHODS
Fifteen specimens of invasive breast cancer - whole tissue sections as well as microdissected tumour cells - were subjected to RLT-PCR. Additionally, IHC and FISH were performed.
RESULTS
Her-2/neu overexpression was detected by FISH and by real-time PCR in the same tumours. In contrast, IHC revealed discordant results.
CONCLUSION
Determination of Her-2/neu amplification by real-time PCR is a sensitive and specific method with some advantages over FISH. This method is simple and reliable and has the potential of categorizing those tumours with borderline Her-2/neu overexpression as determined by IHC.
背景
先前已证明，检测乳腺癌中Her-2/neu过表达具有预后意义。在本研究中，采用基于实时荧光定量PCR（RLT-PCR）的LightCycler-HER-2/neu DNA定量法，并结合免疫组织化学（IHC）和荧光原位杂交（FISH）对乳腺癌中的Her-2/neu表达进行了特征分析。
材料与方法
对15例浸润性乳腺癌标本——全组织切片以及显微切割的肿瘤细胞——进行了RLT-PCR检测。此外，还进行了IHC和FISH检测。
结果
在同一肿瘤中，通过FISH和实时荧光定量PCR均检测到了Her-2/neu过表达。相比之下，IHC结果不一致。
结论
通过实时荧光定量PCR检测Her-2/neu扩增是一种敏感且特异的方法，相较于FISH具有一些优势。该方法简单可靠，有可能对那些经IHC检测为Her-2/neu过表达临界的肿瘤进行分类。
Zotero 插件