细胞表面蛋白的多色成像

Multicolor imaging of cell surface proteins.

作者信息

Vivero-Pol Laura, George Nathalie, Krumm Holger, Johnsson Kai, Johnsson Nils

机构信息

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

出版信息

J Am Chem Soc. 2005 Sep 21;127(37):12770-1. doi: 10.1021/ja0533850.

DOI:10.1021/ja0533850

PMID:16159249

Abstract

We report on a method for the multicolor imaging of cell surface proteins which is based on the labeling of carrier protein (CP) fusion proteins with different fluorophores. In one application, different generations of a cell surface protein can be sequentially labeled to discriminate between old and newly made copies. In another application, fusions to different CPs can be selectively labeled with different fluorophores in one sample. Both applications open up new ways for studying the properties of cell surface proteins of living cells.

摘要

我们报道了一种用于细胞表面蛋白多色成像的方法，该方法基于用不同荧光团标记载体蛋白（CP）融合蛋白。在一种应用中，可以对细胞表面蛋白的不同代次进行顺序标记，以区分旧的和新合成的拷贝。在另一种应用中，可以在一个样本中用不同荧光团对与不同CP的融合蛋白进行选择性标记。这两种应用都为研究活细胞表面蛋白的特性开辟了新途径。