蒙古牛感染问号钩端螺旋体哈焦血清型后感染持续存在的血清学证据。

Serological evidence of the persistence of infection with Leptospira interrogans serovar Hardjo in cattle in Mongolia.

作者信息

Odontsetseg Namsraijav, Sakoda Yoshihiro, Kida Hiroshi

机构信息

Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Sapporo, Hokkaido 060-0818, Japan.

出版信息

Microbiol Immunol. 2005;49(9):865-9. doi: 10.1111/j.1348-0421.2005.tb03676.x.

DOI:10.1111/j.1348-0421.2005.tb03676.x

PMID:16172542

Abstract

Serum samples were collected randomly from Mongol breed cattle in three geographically distinct provinces of Mongolia. Antibodies specific to Leptospira interrogans serovar Hardjo were detected by microscopic agglutination test (MAT) at titres of 100 or more in 80.4% (86/107) of the samples from Dornod Province, but in only 28.9% (13/45) in Arkhangai and 23.5% (12/51) in Khuvsgul Provinces, respectively. Treatment of 9 serum samples from Dornod, positive to serovar Hardjo in MAT and negative in the homologous immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA), with 2-mercaptoethanol (2-ME) indicated that those animals were recently infected.

摘要

从蒙古国三个地理位置不同省份的蒙古牛中随机采集血清样本。通过显微镜凝集试验（MAT）检测，在东方省80.4%（86/107）的样本中，检测到针对问号钩端螺旋体哈焦血清型的抗体效价为100或更高，但在后杭爱省仅为28.9%（13/45），在库苏古尔省仅为23.5%（12/51）。对来自东方省的9份血清样本进行处理，这些样本在MAT中对哈焦血清型呈阳性，而在同源免疫球蛋白G（IgG）酶联免疫吸附测定（ELISA）中呈阴性，用2-巯基乙醇（2-ME）处理表明这些动物最近受到了感染。