[肾上腺髓质素及其受体mRNA在正常肾上腺髓质和嗜铬细胞瘤组织中的表达]

[Expression of adrenomedullin and its receptor mRNA in the tissues of normal adrenal medulla and pheochromocytoma].

作者信息

Liu Dong-Mei, Zeng Zheng-Pei, Li Han-Zhong, Fan Xin-Rong, Liu Guo-Qiang, Yan Wei-Gang, Tong An-Li, Zheng Xin

机构信息

Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.

出版信息

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Aug;27(4):452-6.

PMID:16178438

Abstract

OBJECTIVE

To investigate the expression of human adrenomedullin (ADM) and its receptor-receptor activity modifying protein 2/calcitonin receptor-like receptor (RAMP2/CRLR) mRNA in the tissues of normal adrenal medulla and pheochromocytoma.

METHODS

Total RNA was extracted from normal adrenal medulla and pheochromocytomas. The expression of ADM and RAMP2/CRLR mRNA were studied by reverse transcription-polymerase chain reaction. The ratios of ADM/GAPDH, RAMP2/ GAPDH, CRLR/GAPDH were used to evaluate the expression levels of ADM, RAMP2 and CRLR mRNA.

RESULTS

Expressions of ADM and its receptor- RAMP2/CRLR mRNA were detected in normal adrenal medulla and pheochromocytoma tissues. ADM/GAPDH were 0.48+/-0.09 and 0.75+/-0.24, RAMP2/ GAPDH 0.79+/-0.12 and 1.29+/-0.30, CRLR/GAPDH 0.40+/-0.08 and 0.87+/-0.22 in normal adrenal medulla and pheochromocytomas, respectively (P < 0.05).

CONCLUSION

ADM exerts a possible autocrine or paracrine effect in the adrenal. ADM may be involved in the pathogenesis of pheochromocytoma.

摘要

目的

研究人肾上腺髓质素（ADM）及其受体——受体活性修饰蛋白2/降钙素受体样受体（RAMP2/CRLR）mRNA在正常肾上腺髓质和嗜铬细胞瘤组织中的表达情况。

方法

从正常肾上腺髓质和嗜铬细胞瘤中提取总RNA。采用逆转录-聚合酶链反应研究ADM和RAMP2/CRLR mRNA的表达。用ADM/GAPDH、RAMP2/GAPDH、CRLR/GAPDH的比值评估ADM、RAMP2和CRLR mRNA的表达水平。

结果

在正常肾上腺髓质和嗜铬细胞瘤组织中检测到ADM及其受体RAMP2/CRLR mRNA的表达。正常肾上腺髓质和嗜铬细胞瘤中ADM/GAPDH分别为0.48±0.09和0.75±0.24，RAMP2/GAPDH分别为0.79±0.12和1.29±0.30，CRLR/GAPDH分别为0.40±0.08和0.87±0.22（P<0.05）。