从受污染土壤和沉积物中富集的混合培养物对四氯乙烷、全氯乙烯及其混合物的厌氧转化

Anaerobic transformation of tetrachloroethane, perchloroethylene, and their mixtures by mixed-cultures enriched from contaminated soils and sediments.

作者信息

Aulenta F, Fina A, Potalivo M, Petrangeli Papini M, Rossetti S, Majone M

机构信息

Department of Chemistry, University of Rome "La Sapienza", p.le A. Moro 5, 00185 Rome, Italy.

出版信息

Water Sci Technol. 2005;52(1-2):357-62.

PMID:16180450

Abstract

The focus of this research was to investigate the anaerobic transformation of tetrachloroethane (TeCA), perchloroethylene (PCE), and their mixtures by mixed cultures enriched from contaminated soils or sediments. Batch transformation studies were conducted using TeCA (60 microM), PCE (60 microM), or TeCA + PCE (each added at 60 microM) as electron acceptor(s) and H2 + acetate (each added at 3 mM) or butyrate (3mM) as electron donor(s). A Dehalococcoides spp.-containing, sediment-enrichment dechlorinated PCE rapidly to ethene (ETH) but slowly and incompletely dechlorinated TeCA. Moreover, when present in mixture with PCE, TeCA disrupted the ability of Dehalococcoides to dechlorinate vinyl chloride. In contrast, the soil-enrichment culture was able to completely dechlorinate TeCA and PCE to ETH, both when added as single contaminants and when added as a mixture.

摘要

本研究的重点是调查从受污染土壤或沉积物中富集的混合培养物对四氯乙烷（TeCA）、全氯乙烯（PCE）及其混合物的厌氧转化情况。使用TeCA（60微摩尔）、PCE（60微摩尔）或TeCA + PCE（各添加60微摩尔）作为电子受体，H2 + 乙酸盐（各添加3毫摩尔）或丁酸盐（3毫摩尔）作为电子供体进行批次转化研究。一种含有脱卤球菌属的沉积物富集培养物能迅速将PCE脱氯为乙烯（ETH），但对TeCA的脱氯缓慢且不完全。此外，当TeCA与PCE混合存在时，会破坏脱卤球菌对氯乙烯脱氯的能力。相比之下，土壤富集培养物能够将TeCA和PCE完全脱氯为ETH，无论是作为单一污染物添加还是作为混合物添加。