[生骨注射液对体外培养大鼠成骨细胞血管内皮生长因子mRNA表达的影响]
[Effects of Shenggu injection on mRNA expression of vascular endothelia growth factor in rat osteoblasts in vitro].
作者信息
Cheng Jie, Peng Rui, Yu Yuan, Wang Yong-qing, Zou Yang, Xia Ren-yun
机构信息
Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Chin J Integr Med. 2005 Sep;11(3):191-4. doi: 10.1007/BF02836503.
OBJECTIVE
To study the effects of Shenggu injection (SGI) on mRNA expression of vascular endothelia growth factor (VEGF) in rat osteoblasts in vitro and to explore its possible molecular mechanisms in promoting fracture healing.
METHODS
Rat osteoblasts cultured in vitro were stimulated with SGI according to the protocol. The expression levels of VEGF mRNA in the cells in every group were examined by reverse-transcriptase ploymerase chain reaction (RT-PCR).
RESULTS
When osteoblasts were stimulated with different concentrations of SGI for 5 days, the expression of VEGF mRNA peaked with 1 mg/ml SGI on the 5th day. When treated with 1 mg/ml SGI from the 1st to the 5th day, the expression of VEGF mRNA increased gradually with the increase of culturing time.
CONCLUSION
SGI could promote significantly the expression of VEGF mRNA in rat osteoblasts in vitro. The levels of expression of VEGF mRNA changed along with different concentrations and stimulating time of SGI.
目的
研究生骨注射液（SGI）对体外培养的大鼠成骨细胞血管内皮生长因子（VEGF）mRNA表达的影响，并探讨其促进骨折愈合的可能分子机制。
方法
按照实验方案用SGI刺激体外培养的大鼠成骨细胞。采用逆转录聚合酶链反应（RT-PCR）检测每组细胞中VEGF mRNA的表达水平。
结果
当用不同浓度的SGI刺激成骨细胞5天时，在第5天1mg/ml SGI组VEGF mRNA表达达到峰值。从第1天至第5天用1mg/ml SGI处理时，VEGF mRNA的表达随培养时间的增加而逐渐升高。
结论
SGI能显著促进体外培养的大鼠成骨细胞VEGF mRNA的表达。VEGF mRNA的表达水平随SGI的不同浓度和刺激时间而变化。
Zotero 插件