Seah L K, Wang P, Murukeshan V M, Chao Z X
School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
Forensic Sci Int. 2006 Jul 13;160(2-3):109-14. doi: 10.1016/j.forsciint.2005.08.018. Epub 2005 Sep 22.
Fluorescence lifetime imaging (FLIM) in frequency domain enables the mapping of the spatial distribution of fluorescence lifetimes of a specimen. It has been extensively applied in biology. In this paper, a theoretical analysis for the fluorescence lifetime determination of latent finger mark samples is described, which is followed by the feasibility study of using FLIM in frequency domain for latent finger marks detection. Preliminary experiments are carried out with latent finger marks treated with a fluorescent powder on two different substrates. The resulting fluorescence lifetime image of finger mark revealed a good contrast, and was able to detect the latent finger marks clearly.
频域荧光寿命成像(FLIM)能够绘制样本荧光寿命的空间分布图谱。它已在生物学中得到广泛应用。本文描述了对潜在指纹样本荧光寿命测定的理论分析,随后开展了关于使用频域FLIM进行潜在指纹检测的可行性研究。使用荧光粉处理过的潜在指纹在两种不同基材上进行了初步实验。所得的指纹荧光寿命图像显示出良好的对比度,能够清晰地检测到潜在指纹。