成年雄性大鼠海马对内嗅皮层损伤反应的新型mRNA探针：初步报告。

New mRNA probes for hippocampal responses to entorhinal cortex lesions in the adult male rat: a preliminary report.

作者信息

Day J R, Min B H, Laping N J, Martin G, Osterburg H H, Finch C E

机构信息

Neurogerontology Division, Andrus Gerontology Center, Los Angeles, California.

出版信息

Exp Neurol. 1992 Jul;117(1):97-9. doi: 10.1016/0014-4886(92)90117-9.

DOI:10.1016/0014-4886(92)90117-9

PMID:1618292

Abstract

Three new mRNA responses were found in the hippocampus of the adult male rat after entorhinal cortex lesions (ECL) that induce synaptic reorganization. Hippocampus cDNA libraries were screened by a subtractive hybridization strategy designed to detect ECL-induced mRNAs. Partial sequencing showed clones with similarities to mouse vimentin, ferritin, and polypeptide 7B-2. A sequence similar to mouse SNAP-25 sequence was also detected. Using a mouse SNAP-25 probe, rat SNAP-25 mRNA increased in the hippocampus after ECL.

摘要

在诱导突触重组的内嗅皮层损伤（ECL）后，成年雄性大鼠海马体中发现了三种新的mRNA反应。通过旨在检测ECL诱导的mRNA的消减杂交策略筛选海马体cDNA文库。部分测序显示克隆与小鼠波形蛋白、铁蛋白和多肽7B-2相似。还检测到与小鼠SNAP-25序列相似的序列。使用小鼠SNAP-25探针，ECL后大鼠海马体中的SNAP-25 mRNA增加。