Demeter Kornél, Zádori Anita, Agoston Viktor A, Madarász Emília
Institute of Experimental Medicine of Hungarian Academy of Sciences, Laboratory of Neural Cell Biology, Szigony u. 43, H-1083 Budapest, Hungary.
Neurosci Res. 2005 Nov;53(3):331-42. doi: 10.1016/j.neures.2005.08.003. Epub 2005 Sep 23.
Neural stem cells were suggested to migrate to and invade intracranial gliomas. In the presented studies, interactions of NE-4C embryonic neural stem cells were investigated with C6 and Gl261, LL and U87, glioblastoma cells or with primary astrocytes. Glioma-derived humoral factors did not influence the proliferation of stem cells. NE-4C-derived humoral factors did not alter the proliferation of Gl261 and U87 cells, but increased the mitotic activity of C6 cells and that of astrocytes. In chimera-aggregates, all types of glioma cells co-aggregated with astrocytes, but most of them segregated from stem cells. Complete intercalation of stem and tumour cells was detected only in chimera-aggregates of Gl261 glioma and NE-4C cells. If mixed suspensions of NE-4C and Gl261 cells were injected into the brain, stem cells survived and grew inside the tumour mass. NE-4C stem cells, however, did not migrate towards the tumour, if implanted near to Gl261 tumours established in the adult mouse forebrain. The observations indicate that not all types of stem cells could be used for targeting all sorts of brain tumours.
有研究表明神经干细胞会迁移并侵入颅内胶质瘤。在本研究中，对NE - 4C胚胎神经干细胞与C6、Gl261、LL和U87胶质母细胞瘤细胞或原代星形胶质细胞之间的相互作用进行了研究。胶质瘤衍生的体液因子不影响干细胞的增殖。NE - 4C衍生的体液因子不会改变Gl261和U87细胞的增殖，但会增加C6细胞和星形胶质细胞的有丝分裂活性。在嵌合体聚集体中，所有类型的胶质瘤细胞都与星形胶质细胞共同聚集，但大多数与干细胞分离。仅在Gl261胶质瘤和NE - 4C细胞的嵌合体聚集体中检测到干细胞与肿瘤细胞的完全插入。如果将NE - 4C和Gl261细胞的混合悬液注入大脑，干细胞会在肿瘤块内存活并生长。然而，如果将NE - 4C干细胞植入成年小鼠前脑已形成的Gl261肿瘤附近，它们不会向肿瘤迁移。这些观察结果表明，并非所有类型的干细胞都可用于靶向所有类型的脑肿瘤。
