Köller W, Kindl H
Z Naturforsch C Biosci. 1979 Dec;34(12):1237-42. doi: 10.1515/znc-1979-1227.
Malate synthase is synthesized de novo in the very phase of germination. Its molecular and immunological properties do not differ from those of malate synthase from fully developed cotyledons. Radioactive leucine was administered to dry seeds of cucumber, and its incorporation into proteins of cotyledons was examined after 2 days of germination. The specific radioactivity of malate synthase, purified by immunoprecipitation and electrophoresis on polyacrylamide gel, was only 1/20 the average value of the total albumin fraction. The minimal incorporation documented by the comparatively low specific activity of isolated malate synthase is discussed in relation to the large pool of malate synthase already present in dry seeds.
苹果酸合酶在种子萌发阶段从头合成。其分子和免疫学特性与完全发育的子叶中的苹果酸合酶没有差异。将放射性亮氨酸施用于黄瓜干种子，在萌发2天后检测其在子叶蛋白质中的掺入情况。通过免疫沉淀和聚丙烯酰胺凝胶电泳纯化的苹果酸合酶的比放射性仅为总白蛋白组分平均值的1/20。结合干种子中已经存在的大量苹果酸合酶库，讨论了分离的苹果酸合酶相对较低的比活性所记录的最低掺入情况。
