Yan Xiao-mei, Zou Qing-hua, Zeng Xun, Zhang Jian-zhong
National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Jun;26(6):444-7.
To Investigate the differences of sorbitol fermentation related genes and optimize molecular analysis method for distinguishing an epidemic with nonepidemic strains of Vibrio cholerae.
Sequence analysis on four genes of sugar fermentation stimulation protein, periplasmic maltose-binding protein, periplasmic phosphate-binding protein and periplasmic amino acid-binding protein.
In this study, the following data was noticed: for O1 serogroup El Tor biotype V. cholerae, twenty-four epidemic and eight nonepidemic strains were chosen; For O139 serogroup V. cholerae, five epidemic and four nonepidemic strains were chosen. With those genes of sugar fermentation stimulation protein, there were three point mutations. The 106th, 150th, 378th oligonucleotide in epidemic strains were A, A and T, comparing to the nonepidemic strains which were G, G and C. When comparing the protein sequences, epidemic strains had a Threonine at 36th amino acid, whereas nonepidemic strains had an Alanine. The results in O139 serogroup were consistent with those in O1 serogroup El Tor biotype strains. Another two point mutations were found in the genes of periplasmic maltose-binding protein. The 999th, 1003rd oligonucleotides in epidemic strains were A and C, while in nonepidemic which were G and T. For the gene of periplasmic amino acid-binding protein, two point mutations were noticed. The 504th and 690th oligonucleotides in epidemic strains were T and C, but were C and T in nonepidemic. However, no amino acid differences were found in periplasmic maltose-binding protein and periplasmic amino acid-binding protein. For periplasmic amino acid-binding protein gene, there was no difference on oligonucleotide between epidemic and nonepidemic strains.
Results suggested that SNPs in these genes might serve as a useful tool to distinguish the epidemic strains from nonepidemic strains. The 36th amino acid mutation of sugar fermentation stimulation protein in epidemic and nonepidemic strains might change the activity of the protein which might be associated with sorbitol fermentation.
研究山梨醇发酵相关基因的差异，优化区分霍乱弧菌流行株与非流行株的分子分析方法。
对糖发酵刺激蛋白、周质麦芽糖结合蛋白、周质磷酸盐结合蛋白和周质氨基酸结合蛋白这四个基因进行序列分析。
在本研究中，注意到以下数据：对于O1群埃尔托生物型霍乱弧菌，选取了24株流行株和8株非流行株；对于O139群霍乱弧菌，选取了5株流行株和4株非流行株。在糖发酵刺激蛋白的那些基因中，存在三个点突变。流行株中第106、150、378个寡核苷酸分别为A、A和T，而非流行株为G、G和C。在比较蛋白质序列时，流行株在第36个氨基酸处为苏氨酸，而非流行株为丙氨酸。O139群中的结果与O1群埃尔托生物型菌株中的结果一致。在周质麦芽糖结合蛋白的基因中发现另外两个点突变。流行株中第999、1003个寡核苷酸为A和C，而非流行株为G和T。对于周质氨基酸结合蛋白的基因，注意到两个点突变。流行株中第504和690个寡核苷酸为T和C，而非流行株为C和T。然而，在周质麦芽糖结合蛋白和周质氨基酸结合蛋白中未发现氨基酸差异。对于周质氨基酸结合蛋白基因，流行株与非流行株在寡核苷酸上没有差异。
结果表明，这些基因中的单核苷酸多态性可能是区分流行株与非流行株的有用工具。流行株与非流行株中糖发酵刺激蛋白的第36个氨基酸突变可能改变该蛋白的活性，这可能与山梨醇发酵有关。
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