Meincken M, Holroyd D L, Rautenbach M
UNESCO Associated Centre for Macromolecules, Department of Chemistry, University of Stellenbosch, Matieland, South Africa.
Antimicrob Agents Chemother. 2005 Oct;49(10):4085-92. doi: 10.1128/AAC.49.10.4085-4092.2005.
The influences of the antibacterial magainin 2 and PGLa from the African clawed frog (Xenopus laevis) and the hemolytic bee venom melittin on Escherichia coli as the target cell were studied by atomic force microscopy (AFM). Nanometer-scale images of the effects of the peptides on this gram-negative bacterium's cell envelope were obtained in situ without the use of fixing agents. These high-resolution AFM images of the surviving and intact target cells before and after peptide treatment showed distinct changes in cell envelope morphology as a consequence of peptide action. Although all three peptides are lytic to E. coli, it is clear from this AFM study that each peptide causes distinct morphological changes in the outer membrane and in some cases the inner membrane, probably as a consequence of different mechanisms of action.
利用原子力显微镜（AFM）研究了来自非洲爪蟾（非洲爪蟾）的抗菌肽马盖宁2和PGLa以及具有溶血作用的蜂毒肽蜂毒素对作为靶细胞的大肠杆菌的影响。在不使用固定剂的情况下，原位获得了这些肽对这种革兰氏阴性细菌细胞壁作用的纳米级图像。这些肽处理前后存活且完整的靶细胞的高分辨率AFM图像显示，由于肽的作用，细胞壁形态发生了明显变化。尽管所有三种肽对大肠杆菌都有裂解作用，但从这项AFM研究中可以清楚地看出，每种肽在外膜甚至在某些情况下在内膜上都会引起明显的形态变化，这可能是由于不同的作用机制所致。
