使用一台3.9克便携式双光子荧光微型内窥镜进行活体脑成像。

In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope.

作者信息

Flusberg Benjamin A, Jung Juergen C, Cocker Eric D, Anderson Erik P, Schnitzer Mark J

机构信息

James H. Clark Center for Biomedical Engineering & Sciences, Stanford University, Stanford, California 94305-5435, USA.

出版信息

Opt Lett. 2005 Sep 1;30(17):2272-4. doi: 10.1364/ol.30.002272.

DOI:10.1364/ol.30.002272

PMID:16190441

Abstract

We introduce a compact two-photon fluorescence microendoscope based on a compound gradient refractive index endoscope probe, a DC micromotor for remote adjustment of the image plane, and a flexible photonic bandgap fiber for near distortion-free delivery of ultrashort excitation pulses. The imaging head has a mass of only 3.9 g and provides micrometer-scale resolution. We used portable two-photon microendoscopy to visualize hippocampal blood vessels in the brains of live mice.

摘要

我们介绍了一种紧凑型双光子荧光微型内窥镜，它基于复合梯度折射率内窥镜探头、用于远程调节像平面的直流微电机以及用于近乎无失真地传输超短激发脉冲的柔性光子带隙光纤。成像头的质量仅为3.9克，并提供微米级分辨率。我们使用便携式双光子显微内窥镜观察了活体小鼠大脑中的海马体血管。