达卡巴嗪与基于金纳米颗粒的DNA碱基和寡核苷酸的特异性结合。

Specific binding of dacarbazine to DNA bases and oligonucleotides based on gold nanoparticles.

作者信息

Zhang Renyun, Wang Xuemei, He Nongyue

机构信息

Chien-Shiung Wu Laboratory, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P R. China.

出版信息

J Nanosci Nanotechnol. 2005 Aug;5(8):1245-8. doi: 10.1166/jnn.2005.216.

DOI:10.1166/jnn.2005.216

PMID:16193986

Abstract

We studied the specific binding of an anticancer drug, dacarbazine (DTIC), to DNA bases and oligonucleotides attached to gold nanoparticles by using electrochemical methods, and the results indicate that the presence of gold nanoparticles could facilitate the binding of dacarbazine to specific DNA bases and remarkably enhance the relative detection sensitivity. The results of the study on interaction of dacarbazine with oligonucleotides also illustrate that dacarbazine could recognize some specific sequence in DNA chain and sensitively detect single-base mismatch in DNA helix.

摘要

我们采用电化学方法研究了一种抗癌药物达卡巴嗪（DTIC）与附着在金纳米颗粒上的DNA碱基和寡核苷酸的特异性结合，结果表明金纳米颗粒的存在能够促进达卡巴嗪与特定DNA碱基的结合，并显著提高相对检测灵敏度。达卡巴嗪与寡核苷酸相互作用的研究结果还表明，达卡巴嗪能够识别DNA链中的一些特定序列，并灵敏地检测DNA螺旋中的单碱基错配。